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Marked differences between MARC-145 cells and swine alveolar macrophages in IFNβ-induced activation of antiviral state against PRRSV
- Source :
- Veterinary Immunology and Immunopathology. 139:57-60
- Publication Year :
- 2011
- Publisher :
- Elsevier BV, 2011.
-
Abstract
- The activation of antiviral activity induced by recombinant swine interferon beta (rswIFNβ) against PRRSV was comparatively examined in MARC-145 cells and porcine alveolar macrophages (PAMs). A dose–response analysis showed, in MARC-145 cells, that isolate Mo25544 was highly sensitive to rswIFNβ while a vaccine strain and isolate PDV130-9301 were resistant to different extents. In contrast, all three viruses were equally sensitive to rswIFNβ in PAMs even at the lowest dose of IFN utilized in the bioassays. To analyze potential differences in mechanisms of antiviral activation between these cells, treatment with 2-aminopurine (2-AP), an inhibitor of double-stranded RNA-dependent protein kinase (PKR), was performed in rswIFNβ-treated cells. Addition of 2-AP to rswIFNβ-primed MARC-145 cells restored replication of the Mo25544 isolate, and to some extent that of vaccine virus and PDV130-9301. In contrast, virus replication could not be rescued for any of the three viruses with 2-AP in rswIFNβ-treated PAMs. The differences in sensitivity of PRRSV to rswIFNβ as well as the effects of 2-AP strongly suggest that MARC-145 cells and PAMs utilize different rswIFNβ-associated antiviral pathways. Therefore, studies to understand virus-host cell interactions performed in MARC-145 cells require additional scrutiny when utilized as a host cell model for immunologic responses to PRRSV.
- Subjects :
- Dose-Response Relationship, Drug
General Veterinary
Interferon beta
Swine
Immunology
Cell
Porcine Reproductive and Respiratory Syndrome
Interferon-beta
Biology
Lymphocyte Activation
Virology
Protein kinase R
Cell Line
law.invention
medicine.anatomical_structure
Viral replication
law
Macrophages, Alveolar
medicine
Alveolar macrophage
Recombinant DNA
Animals
Bioassay
Porcine respiratory and reproductive syndrome virus
Protein kinase A
Subjects
Details
- ISSN :
- 01652427
- Volume :
- 139
- Database :
- OpenAIRE
- Journal :
- Veterinary Immunology and Immunopathology
- Accession number :
- edsair.doi.dedup.....631168e65489cfca87abbc0686f511af