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Targeted delivery of hyaluronic acid to the ocular surface by a polymer-peptide conjugate system for dry eye disease
- Source :
- Acta biomaterialia. 55
- Publication Year :
- 2016
-
Abstract
- Hyaluronic acid (HA) solutions effectively lubricate the ocular surface and are used for the relief of dry eye related symptoms. However, HA undergoes rapid clearance due to limited adhesion, which necessitates frequent instillation. Conversely, highly viscous artificial tear formulations with HA blur vision and interfere with blinking. Here, we developed an HA-eye drop formulation that selectively binds and retains HA for extended periods of time on the ocular surface. We synthesized a heterobifunctional polymer-peptide system with one end binding HA while the other end binding either sialic acid-containing glycosylated transmembrane molecules on the ocular surface epithelium, or type I collagen molecule within the tissue matrix. HA solution was mixed with the polymer-peptide system and tested on both ex vivo and in vivo models to determine its ability to prolong HA retention. Furthermore, rabbit ocular surface tissues treated with binding peptides and HA solutions demonstrated superior lubrication with reduced kinetic friction coefficients compared to tissues treated with conventional HA solution. The results suggest that binding peptide-based solution can keep the ocular surface enriched with HA for prolonged times as well as keep it lubricated. Therefore, this system can be further developed into a more effective treatment for dry eye patients than a standard HA eye drop. Statement of Significance Eye drop formulations containing HA are widely used to lubricate the ocular surface and relieve dry eye related symptoms, however its low residence time remains a challenge. We designed a polymer-peptide system for the targeted delivery of HA to the ocular surface using sialic acid or type I collagen as anchors for HA immobilization. The addition of the polymer-peptide system to HA eye drop exhibited a reduced friction coefficient, and it can keep the ocular surface enriched with HA for prolonged time. This system can be further developed into a more effective treatment for dry eye than a standard HA eye drop.
- Subjects :
- Materials science
genetic structures
Stereochemistry
medicine.medical_treatment
Biomedical Engineering
02 engineering and technology
Eye
Biochemistry
Biomaterials
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Drug Delivery Systems
In vivo
Hyaluronic acid
medicine
Animals
Humans
Hyaluronic Acid
Molecular Biology
Eye drop
General Medicine
021001 nanoscience & nanotechnology
eye diseases
Sialic acid
Blurring vision
Artificial tears
chemistry
030221 ophthalmology & optometry
Biophysics
Dry Eye Syndromes
sense organs
0210 nano-technology
Peptides
Ex vivo
Type I collagen
Biotechnology
Subjects
Details
- ISSN :
- 18787568
- Volume :
- 55
- Database :
- OpenAIRE
- Journal :
- Acta biomaterialia
- Accession number :
- edsair.doi.dedup.....630a519a14bb9cf102a00913f58ae5fb