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Inhibitory effect on in vitro Streptococcus oralis biofilm of a soda-lime glass containing silver nanoparticles coating on titanium alloy
- Source :
- Scopus, RUO. Repositorio Institucional de la Universidad de Oviedo, instname, PLoS ONE, Digital.CSIC. Repositorio Institucional del CSIC, PLoS ONE, Vol 7, Iss 8, p e42393 (2012)
- Publication Year :
- 2012
- Publisher :
- Public Library of Science, 2012.
-
Abstract
- This is an open-access article.-- et al.<br />This paper reports the effect of soda-lime-glass-nAg coating on the viability of an in vitro biofilm of Streptococcus oralis. Three strains (ATCC 35037 and two clinical isolates from periodontitis patients) were grown on coated with glass, glass containing silver nanoparticles, and uncoated titanium alloy disks. Two different methods were used to quantify biofilm formation abilities: crystal violet staining and determination of viable counts. The influence of the surface morphology on the cell attachment was studied. The surface morphology was characterized by scanning electron microscopy (SEM) and using a profilometer. SEM was also used to study the formation and the development of biofilm on the coated and uncoated disks. At least a >99.7% inocula reduction of biofilm respect to titanium disks and also to glass coated disks was observed in the glass-nAg coated disks for all the studied strains. A quantitative evaluation of the release of silver was conducted in vitro to test whether and to what extend the biocidal agent (silver) could leach from the coating. These findings suggest that the biofilm formation of S. oralis strains is highly inhibited by the glass-nAg and may be useful for materials which require durable antibacterial effect on their surfaces, as it is the case of dental implants. © 2012 Cabal et al.<br />This work was supported by the Spanish Ministry of Science and Innovation under the project MAT2009-14542-C02-01.
- Subjects :
- Soda-lime glass
Scanning electron microscope
lcsh:Medicine
Metal Nanoparticles
Glass Science
Bacterial Adhesion
Silver nanoparticle
chemistry.chemical_compound
Oral Diseases
Coating
Nanotechnology
lcsh:Science
Titanium
Multidisciplinary
biology
Temperature
Oxides
Plankton
Metals
Medicine
Material by Structure
Research Article
Biotechnology
Silver
Materials science
Surface Properties
Materials Science
Oral Medicine
chemistry.chemical_element
engineering.material
Microbiology
Biomaterials
Alloys
Humans
Sodium Hydroxide
Crystal violet
Biology
Nanomaterials
Vitreous Materials
Staining and Labeling
lcsh:R
Biofilm
Streptococcus oralis
Calcium Compounds
equipment and supplies
biology.organism_classification
chemistry
Dentistry
Biofilms
Bionanotechnology
engineering
lcsh:Q
Gentian Violet
Glass
Nuclear chemistry
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Scopus, RUO. Repositorio Institucional de la Universidad de Oviedo, instname, PLoS ONE, Digital.CSIC. Repositorio Institucional del CSIC, PLoS ONE, Vol 7, Iss 8, p e42393 (2012)
- Accession number :
- edsair.doi.dedup.....62ffb9552544c0136dfc6308c37f0e88