Back to Search
Start Over
Elucidating potentially significant genomic regions involved in the initiation and progression of undifferentiated pleomorphic sarcoma
- Source :
- Rare Tumors, Rare Tumors, Vol 5, Iss 1, Pp e14-e14 (2013)
- Publication Year :
- 2013
- Publisher :
- PAGEPress Publications, 2013.
-
Abstract
- Sarcomas are cancers that arise in soft tissues or bone and make up a small percentage of malignancies. In an effort to identify potential genetic targets for therapy, this study explores the genomic landscape of a metastatic undifferentiated pleomorphic sarcoma (UPS) with spindle cell morphology. Thick sections (50 µm) of formalin-fixed, paraffin-embedded tissue from a primary, recurrent, and metastatic tumor were collected and processed from a single patient for DNA content-based flow-sorting and analyses. Nuclei of diploid and aneuploid populations were sorted from the malignant tissues and their genomes interrogated with array comparative genomic hybridization. The third sample was highly degraded and did not contain any intact ploidy peaks in our flow assays. A 2.5N aneuploid population was identified in the primary and recurrent sample. We detected a series of shared and unique genomic aberrations in the sorted aneuploid populations. The patterns of aberrations suggest that two similar but independent clonal populations arose during the clinical history of this rare tumor. None of these aberrations were detected in the matching sorted diploid samples. The targeted regions of interest might play a role in UPS and may lead to clinical significance with further investigation.
- Subjects :
- Pathology
medicine.medical_specialty
education.field_of_study
Histology
flow-sort cytometry
Population
Biology
Cell morphology
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Genome
lcsh:RC254-282
Undifferentiated Pleomorphic Sarcoma
Article
undifferentiated pleomorphic sarcoma
Oncology
Metastatic Undifferentiated Pleomorphic Sarcoma
medicine
Clinical significance
array comparative genomic hybridization
Ploidy
education
undifferentiated pleomorphic sarcoma, flow-sort cytometry, array comparative genomic hybridization
Comparative genomic hybridization
Subjects
Details
- Language :
- English
- ISSN :
- 20363613 and 20363605
- Volume :
- 5
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Rare Tumors
- Accession number :
- edsair.doi.dedup.....62ecd05b6866390a36c57ca9b5925ba4