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Endothelium-derived nitric oxide supports renin cell recruitment through the nitric oxide-sensitive guanylate cyclase pathway
- Source :
- Hypertension (Dallas, Tex. : 1979). 61(2)
- Publication Year :
- 2013
-
Abstract
- Chronic challenge of renin–angiotensin causes recruitment of renin-producing cells in the kidney along the media layer of afferent arterioles and hypertrophy of cells in the juxtaglomerular apparatus. This study aimed to define the role of nitric oxide (NO) with regard to the recruitment pattern of renin-producing cells and to the possible pathways along which NO could act. We considered the hypothesis that endothelium-derived NO acts via NO-sensitive guanylate cyclase. Mice were treated with low-salt diet in combination with the angiotensin I–converting enzyme inhibitor enalapril for 3 weeks, which led to a 13-fold increase in renin expression associated with marked recruitment of renin cells in afferent arterioles and hypertrophy of the juxtaglomerular apparatus in wild-type mice. In wild-type mice additionally treated with the nonselective NO synthase inhibitor L-NAME, the recruitment of renin-expressing cells along the afferent arterioles was absent and juxtaglomerular hypertrophy was diminished. An almost identical attenuation of renin cell recruitment as with L-NAME treatment in wild-type mice was found in mice lacking the endothelial isoform of NO synthase. Treatment of mice lacking NO-sensitive guanylate cyclase in renin-expressing cells and preglomerular smooth muscle cells with low-salt diet in combination with the angiotensin I–converting enzyme inhibitor enalapril for 3 weeks produced juxtaglomerular hypertrophy like in wild-type mice, but no recruitment in afferent arterioles. These findings suggest that endothelium-derived NO and concomitant formation of cGMP in preglomerular renin cell precursors supports recruitment of renin-expressing cells along preglomerular vessels, but not in the juxtaglomerular apparatus.
- Subjects :
- medicine.medical_specialty
Afferent arterioles
Endothelium
Nitric Oxide Synthase Type III
Blood Pressure
Biology
Kidney
Nitric Oxide
Article
Nitric oxide
Muscle hypertrophy
chemistry.chemical_compound
Mice
Internal medicine
Renin–angiotensin system
Renin
Internal Medicine
medicine
Animals
Enzyme Inhibitors
Mice, Knockout
urogenital system
Juxtaglomerular apparatus
Juxtaglomerular Apparatus
medicine.anatomical_structure
Endocrinology
NG-Nitroarginine Methyl Ester
chemistry
Guanylate Cyclase
Endothelium, Vascular
Signal Transduction
Subjects
Details
- ISSN :
- 15244563
- Volume :
- 61
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Hypertension (Dallas, Tex. : 1979)
- Accession number :
- edsair.doi.dedup.....62cd1bbc817ab136c752d92b7147fd48