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A Long-Term Conserved Satellite DNA That Remains Unexpanded in Several Genomes of Characiformes Fish Is Actively Transcribed
- Source :
- Genome Biology and Evolution, Scopus, Repositório Institucional da UNESP, Universidade Estadual Paulista (UNESP), instacron:UNESP
- Publication Year :
- 2021
- Publisher :
- Uppsala universitet, Systematisk biologi, 2021.
-
Abstract
- Made available in DSpace on 2021-06-25T10:54:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-02-03 Eukaryotic genomes contain large amounts of repetitive DNA sequences, such as tandemly repeated satellite DNAs (satDNAs). These sequences are highly dynamic and tend to be genus- or species-specific due to their particular evolutionary pathways, although there are few unusual cases of conserved satDNAs over long periods of time. Here, we used multiple approaches to reveal that an satDNA named CharSat01-52 originated in the last common ancestor of Characoidei fish, a superfamily within the Characiformes order, ∼140-78 Ma, whereas its nucleotide composition has remained considerably conserved in several taxa. We show that 14 distantly related species within Characoidei share the presence of this satDNA, which is highly amplified and clustered in subtelomeric regions in a single species (Characidium gomesi), while remained organized as small clusters in all the other species. Defying predictions of the molecular drive of satellite evolution, CharSat01-52 shows similar values of intra- and interspecific divergence. Although we did not provide evidence for a specific functional role of CharSat01-52, its transcriptional activity was demonstrated in different species. In addition, we identified short tandem arrays of CharSat01-52 embedded within single-molecule real-time long reads of Astyanax paranae (536 bp-3.1 kb) and A. mexicanus (501 bp-3.9 kb). Such arrays consisted of head-to-tail repeats and could be found interspersed with other sequences, inverted sequences, or neighbored by other satellites. Our results provide a detailed characterization of an old and conserved satDNA, challenging general predictions of satDNA evolution. Departamento de Ciências Biológicas Faculdade de Ciências Universidade Estadual Paulista UNESP Campus de Bauru Departamento de Biologia Estrutural e Funcional Instituto de Biociências de Botucatu Universidade Estadual Paulista UNESP Department of Organismal Biology-Systematic Biology Evolutionary Biology Centre Uppsala University Departamento de Genética Instituto de Ciências Biológicas e da Saúde ICBS Universidade Federal Rural do Rio de Janeiro Rio de Janerio Departamento de Ciências Biológicas Faculdade de Ciências Universidade Estadual Paulista UNESP Campus de Bauru Departamento de Biologia Estrutural e Funcional Instituto de Biociências de Botucatu Universidade Estadual Paulista UNESP
- Subjects :
- 0106 biological sciences
Most recent common ancestor
AcademicSubjects/SCI01140
Transcription, Genetic
Satellite DNA
Biology
Characiformes
DNA, Satellite
010603 evolutionary biology
01 natural sciences
Genome
Evolution, Molecular
Evolutionsbiologi
03 medical and health sciences
Tandem repeat
Genetics
Animals
Repeated sequence
Ecology, Evolution, Behavior and Systematics
Conserved Sequence
030304 developmental biology
neotropical fish
0303 health sciences
Evolutionary Biology
Base Sequence
repetitive DNA
AcademicSubjects/SCI01130
Genetic Variation
Subtelomere
biology.organism_classification
Molecular drive
tandem repeats
Evolutionary biology
satDNA
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Genome Biology and Evolution, Scopus, Repositório Institucional da UNESP, Universidade Estadual Paulista (UNESP), instacron:UNESP
- Accession number :
- edsair.doi.dedup.....62cc74dc413f5f6677484f4f87838d37