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Molecular cloning of human squamous cell carcinoma antigen 1 gene and characterization of its promoter

Molecular cloning of human squamous cell carcinoma antigen 1 gene and characterization of its promoter

Authors :
Hiroto Shinomiya
Koji Hashimoto
Mari Iwamoto
Masaharu Ito
Toshimasa Kihana
Susumu Hirose
Satoru Kyo
Yasushi Hanakawa
Katsuyuki Hamada
Yoshihiro Asano
Source :
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1518:124-131
Publication Year :
2001
Publisher :
Elsevier BV, 2001.

Abstract

The squamous cell carcinoma antigen (SCCA) serves as a serological marker for squamous cell carcinomas. Molecular cloning of the SCCA genomic region has revealed the presence of two tandemly arrayed genes, SCCA1 and SCCA2 , which are 95% identical in nucleotide sequence. SCCA1 is a papain-like cysteine proteinase inhibitor, while SCCA2 is a chymotrypsin-like serine proteinase inhibitor. We analyzed here the sequence and the promoter activity of the 5′-flanking region of the SCCA1 gene. Deletion analysis of SCCA1 and SCCA2 promoter identified a 471-bp core promoter region upstream of the transcription start site. The transcriptional activity of SCCA1 promoter was up-regulated in squamous cell carcinoma cells, compared with keratinocyte and adenocarcinoma cells. The ratios of SCCA1 to SCCA2 promoter activity in squamous cell carcinoma, keratinocyte and adenocarcinoma cells were respectively 1.6, 5.3 and 2.8. Position −50 of SCCA1 and SCCA2 promoters played an important role in determining the promoter activities of SCCA1 and SCCA2 . These findings suggest that the transcriptional regulation of SCCA1 and SCCA2 might differ among squamous cell carcinoma, keratinocyte and adenocarcinoma cells, and that SCCA1 promoter might be a potential target of gene therapy for squamous cell carcinoma.

Details

ISSN :
01674781
Volume :
1518
Database :
OpenAIRE
Journal :
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression
Accession number :
edsair.doi.dedup.....62c26e79dcf906ca0cf6ec716ffab9ba
Full Text :
https://doi.org/10.1016/s0167-4781(01)00174-9