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Molecular cloning of human squamous cell carcinoma antigen 1 gene and characterization of its promoter
Molecular cloning of human squamous cell carcinoma antigen 1 gene and characterization of its promoter
- Source :
- Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1518:124-131
- Publication Year :
- 2001
- Publisher :
- Elsevier BV, 2001.
-
Abstract
- The squamous cell carcinoma antigen (SCCA) serves as a serological marker for squamous cell carcinomas. Molecular cloning of the SCCA genomic region has revealed the presence of two tandemly arrayed genes, SCCA1 and SCCA2 , which are 95% identical in nucleotide sequence. SCCA1 is a papain-like cysteine proteinase inhibitor, while SCCA2 is a chymotrypsin-like serine proteinase inhibitor. We analyzed here the sequence and the promoter activity of the 5′-flanking region of the SCCA1 gene. Deletion analysis of SCCA1 and SCCA2 promoter identified a 471-bp core promoter region upstream of the transcription start site. The transcriptional activity of SCCA1 promoter was up-regulated in squamous cell carcinoma cells, compared with keratinocyte and adenocarcinoma cells. The ratios of SCCA1 to SCCA2 promoter activity in squamous cell carcinoma, keratinocyte and adenocarcinoma cells were respectively 1.6, 5.3 and 2.8. Position −50 of SCCA1 and SCCA2 promoters played an important role in determining the promoter activities of SCCA1 and SCCA2 . These findings suggest that the transcriptional regulation of SCCA1 and SCCA2 might differ among squamous cell carcinoma, keratinocyte and adenocarcinoma cells, and that SCCA1 promoter might be a potential target of gene therapy for squamous cell carcinoma.
- Subjects :
- Transcriptional Activation
Genetic enhancement
Molecular Sequence Data
Cell
Biophysics
Uterine Cervical Neoplasms
Biology
Biochemistry
Antigens, Neoplasm
Structural Biology
Tumor Cells, Cultured
Genetics
medicine
Carcinoma
Transcriptional regulation
Humans
Cloning, Molecular
Promoter Regions, Genetic
Gene
Serpins
Base Sequence
Promoter
medicine.disease
Molecular biology
medicine.anatomical_structure
Carcinoma, Squamous Cell
Cancer research
Adenocarcinoma
Female
Keratinocyte
Subjects
Details
- ISSN :
- 01674781
- Volume :
- 1518
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression
- Accession number :
- edsair.doi.dedup.....62c26e79dcf906ca0cf6ec716ffab9ba
- Full Text :
- https://doi.org/10.1016/s0167-4781(01)00174-9