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Ni-Nitrilotriacetic Acid Affinity SELEX Method for Selection of DNA Aptamers Specific to the N-Cadherin Protein
- Source :
- ACS combinatorial science. 22(12)
- Publication Year :
- 2020
-
Abstract
- Nucleic acid aptamers are single-stranded oligonucleotides that may be evolved for affinity and specificity for their targets and can be easily produced, regenerated, and stabilized. In this study, we adapted Ni-NTA (nickle-charged nitrilotriacetic acid) affinity-chromatography in the development of single-stranded DNA aptamers against N-cadherin protein by systematic evolution of ligands by exponential enrichment (SELEX). After ten rounds of selection, two aptamers, designated NS13 and NC23, were selected, which showed low dissociation constants of 93 and 174 nM, respectively. The 5'-carboxyfluorescein-labeled NS13 was used for the sensitive detection of N-cadherin protein by the enzyme-linked oligonucleotide assay (ELONA) method.
- Subjects :
- Nitrilotriacetic Acid
Oligonucleotide
Cadherin
Aptamer
SELEX Aptamer Technique
Nitrilotriacetic acid
General Chemistry
General Medicine
DNA Aptamers
Aptamers, Nucleotide
Cadherins
Ligands
Dissociation constant
chemistry.chemical_compound
chemistry
Biochemistry
Nucleic acid
Organometallic Compounds
Systematic evolution of ligands by exponential enrichment
Subjects
Details
- ISSN :
- 21568944
- Volume :
- 22
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- ACS combinatorial science
- Accession number :
- edsair.doi.dedup.....62bcd60b17c34faf8bc9bc059baeb9ca