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Characterization of different fad-dependent glucose dehydrogenases for possible use in glucose-based biosensors and biofuel cells
- Source :
- Analytical and Bioanalytical Chemistry
- Publication Year :
- 2012
- Publisher :
- Springer Nature, 2012.
-
Abstract
- In this study, different flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenases (FADGDHs) were characterized electrochemically after “wiring” them with an osmium redox polymer [Os(4,4′-dimethyl-2,2′-bipyridine)2(PVI)10Cl]+ on graphite electrodes. One tested FADGDH was that recently discovered in Glomerella cingulata (GcGDH), another was the recombinant form expressed in Pichia pastoris (rGcGDH), and the third was a commercially available glycosylated enzyme from Aspergillus sp. (AspGDH). The performance of the Os-polymer “wired” GDHs on graphite electrodes was tested with glucose as the substrate. Optimal operational conditions and analytical characteristics like sensitivity, linear ranges and current density of the different FADGDHs were determined. The performance of all three types of FADGDHs was studied at physiological conditions (pH 7.4). The current densities measured at a 20 mM glucose concentration were 494 ± 17, 370 ± 24, and 389 ± 19 μA cm−2 for GcGDH, rGcGDH, and AspGDH, respectively. The sensitivities towards glucose were 2.16, 1.90, and 1.42 μA mM−1 for GcGDH, rGcGDH, and AspGDH, respectively. Additionally, deglycosylated rGcGDH (dgrGcGDH) was investigated to see whether the reduced glycosylation would have an effect, e.g., a higher current density, which was indeed found. GcGDH/Os-polymer modified electrodes were also used and investigated for their selectivity for a number of different sugars. Figure Comparison of different parameters for GDHs/Os-polymer modified electrodes
- Subjects :
- Bioelectric Energy Sources
Polymers
fad
Glucose Dehydrogenases
deglycosylation
Biosensing Techniques
02 engineering and technology
7. Clean energy
01 natural sciences
Biochemistry
Analytical Chemistry
chemistry.chemical_compound
Bipyridine
direct electron-transfer
Glucose dehydrogenase
Organic chemistry
redox polymers
cellobiose dehydrogenase
Flavin adenine dinucleotide
glucose dehydrogenase
glucose biosensor
adenine-dinucleotide
biology
oxidase
Osmium
021001 nanoscience & nanotechnology
catalytic-oxidation
Recombinant Proteins
Aspergillus
biofuel cell
Flavin-Adenine Dinucleotide
Graphite
0210 nano-technology
Oxidation-Reduction
Cellobiose dehydrogenase
graphite-electrodes
010402 general chemistry
Sensitivity and Specificity
Redox
Pichia pastoris
carbon electrodes
os-polymer
Original Paper
Chromatography
amperometric biosensors
Substrate (chemistry)
biology.organism_classification
0104 chemical sciences
Glucose
chemistry
Phyllachorales
Biosensor
enzymatic fuel-cells
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Analytical and Bioanalytical Chemistry
- Accession number :
- edsair.doi.dedup.....62b26bfaf85942127dadcb6cdc431cf0