Back to Search
Start Over
Divergent Expression of SPARC, SPARC-L, and SCPP Genes During Jawed Vertebrate Cartilage Mineralization
- Source :
- Frontiers in Genetics, Frontiers in Genetics, Vol 12 (2021)
- Publication Year :
- 2021
- Publisher :
- Frontiers Media S.A., 2021.
-
Abstract
- While cartilage is an ancient tissue found both in protostomes and deuterostomes, its mineralization evolved more recently, within the vertebrate lineage. SPARC, SPARC-L, and the SCPP members (Secretory Calcium-binding PhosphoProtein genes which evolved from SPARC-L) are major players of dentine and bone mineralization, but their involvement in the emergence of the vertebrate mineralized cartilage remains unclear. We performed in situ hybridization on mineralizing cartilaginous skeletal elements of the frog Xenopus tropicalis (Xt) and the shark Scyliorhinus canicula (Sc) to examine the expression of SPARC (present in both species), SPARC-L (present in Sc only) and the SCPP members (present in Xt only). We show that while mineralizing cartilage expresses SPARC (but not SPARC-L) in Sc, it expresses the SCPP genes (but not SPARC) in Xt, and propose two possible evolutionary scenarios to explain these opposite expression patterns. In spite of these genetic divergences, our data draw the attention on an overlooked and evolutionarily conserved peripheral cartilage subdomain expressing SPARC or the SCPP genes and exhibiting a high propensity to mineralize.
- Subjects :
- Lineage (genetic)
Xenopus
In situ hybridization
QH426-470
Scyliorhinus canicula
biology.animal
medicine
Genetics
Xenopus tropicalis
vertebrate evolution
Gene
Genetics (clinical)
Original Research
SCPP
biology
Cartilage
Vertebrate
SPARC
biology.organism_classification
medicine.disease
cartilage mineralization
Cell biology
medicine.anatomical_structure
Phosphoprotein
Molecular Medicine
SPARC-L
Calcification
Subjects
Details
- Language :
- English
- ISSN :
- 16648021
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Frontiers in Genetics
- Accession number :
- edsair.doi.dedup.....62b075ac3deff7813e0a64a1a417abb3