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Elasticity of human embryonic stem cells as determined by atomic force microscopy
- Source :
- Journal of biomechanical engineering. 133(10)
- Publication Year :
- 2011
-
Abstract
- The expansive growth and differentiation potential of human embryonic stem cells (hESCs) make them a promising source of cells for regenerative medicine. However, this promise is off set by the propensity for spontaneous or uncontrolled differentiation to result in heterogeneous cell populations. Cell elasticity has recently been shown to characterize particular cell phenotypes, with undifferentiated and differentiated cells sometimes showing significant differences in their elasticities. In this study, we determined the Young’s modulus of hESCs by atomic force microscopy using a pyramidal tip. Using this method we are able to take point measurements of elasticity at multiple locations on a single cell, allowing local variations due to cell structure to be identified. We found considerable differences in the elasticity of the analyzed hESCs, reflected by a broad range of Young’s modulus (0.05-10 kPa). This surprisingly high variation suggests that elasticity could serve as the basis of a simple and efficient large scale purification/separation technique to discriminate subpopulations of hESCs.
- Subjects :
- Matrigel
Chemistry
Cellular differentiation
Biomedical Engineering
Cell Differentiation
Fibroblasts
Microscopy, Atomic Force
Embryonic stem cell
Regenerative medicine
Elasticity
Cell Line
Drug Combinations
Tissue engineering
Cell culture
Physiology (medical)
Elastic Modulus
Biophysics
Humans
Proteoglycans
Collagen
Laminin
Stem cell
Elasticity (economics)
Embryonic Stem Cells
Biomedical engineering
Subjects
Details
- ISSN :
- 15288951
- Volume :
- 133
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Journal of biomechanical engineering
- Accession number :
- edsair.doi.dedup.....6299bf3f59f3646813465e1f6ef84003