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Biocompatibility of amine-functionalized silica nanoparticles: The role of surface coverage
- Source :
- Small 15:1805400 (2019)
- Publication Year :
- 2019
- Publisher :
- Wiley-v C H Verlag Gmbh, 2019.
-
Abstract
- Here, amorphous silica nanoparticles (NPs), one of the most abundant nanomaterials, are used as an example to illustrate the utmost importance of surface coverage by functional groups which critically determines biocompatibility. Silica NPs are functionalized with increasing amounts of amino groups, and the number of surface exposed groups is quantified and characterized by detailed NMR and fluorescamine binding studies. Subsequent biocompatibility studies in the absence of serum demonstrate that, irrespective of surface modification, both plain and amine-modified silica NPs trigger cell death in RAW 264.7 macrophages. The in vitro results can be confirmed in vivo and are predictive for the inflammatory potential in murine lungs. In the presence of serum proteins, on the other hand, a replacement of only 10% of surface-active silanol groups by amines is sufficient to suppress cytotoxicity, emphasizing the relevance of exposure conditions. Mechanistic investigations identify a key role of lysosomal injury for cytotoxicity only in the presence, but not in the absence, of serum proteins. In conclusion, this work shows the critical need to rigorously characterize the surface coverage of NPs by their constituent functional groups, as well as the impact of serum, to reliably establish quantitative nanostructure activity relationships and develop safe nanomaterials.
- Subjects :
- Biocompatibility
Nanoparticle
02 engineering and technology
General Chemistry
010402 general chemistry
021001 nanoscience & nanotechnology
Fluorescamine
01 natural sciences
Blood proteins
0104 chemical sciences
Nanomaterials
Biomaterials
Silanol
chemistry.chemical_compound
Cytotoxicity
Macrophages
Silica Nanoparticles
Surface-characterization
Surface-functionalization
chemistry
Biophysics
Surface modification
General Materials Science
0210 nano-technology
Biotechnology
Subjects
Details
- Language :
- German
- Database :
- OpenAIRE
- Journal :
- Small 15:1805400 (2019)
- Accession number :
- edsair.doi.dedup.....62831615e33b8f9f04aa8abdff33f9be