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First isolation and genotyping of viruses from recent outbreaks of viral haemorrhagic septicaemia (VHS) in Slovenia
- Source :
- Diseases of aquatic organisms. 92(1)
- Publication Year :
- 2010
-
Abstract
- In November and December 2007, the virus causing viral haemorrhagic septicaemia (VHS) was detected in rainbow trout Oncorhynchus mykiss from 2 fish farms in Slovenia. During 2008 and 2009 the infection spread only among rainbow trout farms and 4 new outbreaks were con- firmed. High mortality and clinical signs of VHS were observed among the diseased fish. VHSV was confirmed by virus isolation, immunoperoxidase test, reverse transcriptase polymerase chain reaction (RT-PCR) and phylogenetic analysis. Based on 1 complete (1524 nucleotides (nt)) and 9 partial (600 nt) glycoprotein gene nucleotide sequences, 9 VHSV isolates from the 6 VHS outbreaks were genetically closely related (99 to 100% identity), and were classified into the Subgroup I-a of Geno- type I, most closely related to the German isolates Dstg21-07, Dstg36-06, and Dstg54-1-07 (99 to 100% identity). Phylogenetic analysis and epidemiological investigations confirmed that the VHS virus had been (re)introduced with imported live fish, and that subsequent outbreaks were linked to the initial infection. Our study shows that direct nucleotide sequencing of RT-PCR products, ampli- fied from the tissue of VHSV-infected fish, represents a reliable tool for fast routine genotyping in diagnostic laboratories. This is the first report of a natural epidemic associated with VHSV infection in Slovenia since the eradication of the disease in 1977.
- Subjects :
- medicine.medical_specialty
Genotype
Fish farming
Slovenia
Outbreak
Aquatic Science
Biology
Virology
Virus
law.invention
Disease Outbreaks
Novirhabdovirus
law
Molecular genetics
Oncorhynchus mykiss
medicine
Hemorrhagic Septicemia, Viral
Animals
Rainbow trout
Genotyping
Ecology, Evolution, Behavior and Systematics
Polymerase chain reaction
Phylogeny
Subjects
Details
- ISSN :
- 01775103
- Volume :
- 92
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Diseases of aquatic organisms
- Accession number :
- edsair.doi.dedup.....6277b6742651f88a6c7956577d44b571