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The Tailless Icosahedral Membrane Virus PRD1 Localizes the Proteins Involved in Genome Packaging and Injection at a Unique Vertex
- Source :
- Journal of Virology. 77:7863-7871
- Publication Year :
- 2003
- Publisher :
- American Society for Microbiology, 2003.
-
Abstract
- The double-stranded DNA (dsDNA) virus PRD1 carries its genome in a membrane surrounded by an icosahedral protein shell. The shell contains 240 copies of the trimeric P3 protein arranged with a pseudo T = 25 triangulation that is reminiscent of the mammalian adenovirus. DNA packaging and infection are believed to occur through the vertices of the particle. We have used immunolabeling to define the distribution of proteins on the virion surface. Antibodies to protein P3 labeled the entire surface of the virus. Most of the 12 vertices labeled with antibodies directed against proteins P5, P2, and P31. These proteins are known to function in virus binding to the cell surface. Proteins P6, P11, and P20 were found on a single vertex per virion. The P6 and P20 proteins are believed to function in DNA packaging. Protein P11 is a pilot protein that is involved in a complex that mediates the early stages of DNA entry to the host cell. Labeling with antibodies to P5 or P2 did not affect the labeling of P6, the unique vertex protein. Labeling with antibodies to the unique vertex protein P6 interfered with the labeling by antibodies to the unique vertex protein P20. We conclude that PRD1 utilizes 11 of its vertices for initial receptor binding. It utilizes a single, unique vertex for both DNA packing during assembly and DNA delivery during infection.
- Subjects :
- Immunology
Genome, Viral
Microbiology
Virus
Single-stranded binding protein
Viral Proteins
03 medical and health sciences
chemistry.chemical_compound
Immunolabeling
Capsid
Virology
Bacteriophage PRD1
030304 developmental biology
0303 health sciences
biology
Structure and Assembly
Virus Assembly
Cryoelectron Microscopy
030302 biochemistry & molecular biology
Virion
Membrane Proteins
Intracellular Membranes
Viral membrane
Molecular biology
3. Good health
Vertex (geometry)
Cell biology
Membrane protein
chemistry
Insect Science
biology.protein
DNA
Subjects
Details
- ISSN :
- 10985514 and 0022538X
- Volume :
- 77
- Database :
- OpenAIRE
- Journal :
- Journal of Virology
- Accession number :
- edsair.doi.dedup.....6271fd23248b356ca1a1cd73206f0d0f