Generation of WAe001-A-58 human embryonic stem cell line with inducible expression of the SARS-CoV-2 nucleocapsid protein

Highlights • A human embryonic stem cell line (WAe001-A-58) with inducible expression of the SARS-CoV-2 nucleocapsid protein was generated by piggyBac transposon system. • This single cell-derived cell line maintained its pluripotency, differentiation potentials, normal morphology and karyotype. • SARS-CoV-2 nucleocapsid protein in this cell line is inducible in a doxycycline dose-dependent manner.
Excessive prostaglandin E2 (PGE2) is the key pathological basis for COVID-19 and a Celebrex treatment of hospitalized COVID-19 patients with comorbidities led to 100% discharged rate and zero death (Hong et al. 2020). It is also suggested that SARS-CoV-2 infected multiple organs and the SARS-CoV nucleocapsid (N) protein transcriptionally drives the expression of the host COX-2 gene. In order to test whether SARS-CoV-2 N protein activates COX-2 transcription in multiple human relevant cell types, an expression inducible human embryonic stem cell line was generated by piggyBac transposon system. This cell line maintained its pluripotency, differentiation potentials, normal morphology and karyotype.