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Transfer RNA Methyltransferases from Thermoplasma acidophilum, a Thermoacidophilic Archaeon
- Source :
- International Journal of Molecular Sciences, Vol 16, Iss 1, Pp 91-113 (2014), International Journal of Molecular Sciences, Volume 16, Issue 1, Pages 91-113
- Publication Year :
- 2014
- Publisher :
- MDPI AG, 2014.
-
Abstract
- We investigated tRNA methyltransferase activities in crude cell extracts from the thermoacidophilic archaeon Thermoplasma acidophilum. We analyzed the modified nucleosides in native initiator and elongator tRNAMet, predicted the candidate genes for the tRNA methyltransferases on the basis of the tRNAMet and tRNALeu sequences, and characterized Trm5, Trm1 and Trm56 by purifying recombinant proteins. We found that the Ta0997, Ta0931, and Ta0836 genes of T. acidophilum encode Trm1, Trm56 and Trm5, respectively. Initiator tRNAMet from T. acidophilum strain HO-62 contained G+, m1I, and m22G, which were not reported previously in this tRNA, and the m2G26 and m22G26 were formed by Trm1. In the case of elongator tRNAMet, our analysis showed that the previously unidentified G modification at position 26 was a mixture of m2G and m22G, and that they were also generated by Trm1. Furthermore, purified Trm1 and Trm56 could methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. However, the speed of methyl-transfer by Trm56 to the precursor RNA was considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed. Moreover, cellular arrangements of the tRNA methyltransferases in T. acidophilum are discussed.
- Subjects :
- Methyltransferase
Thermoplasma
archaea
Archaeal Proteins
Article
Catalysis
Inorganic Chemistry
lcsh:Chemistry
Physical and Theoretical Chemistry
Molecular Biology
lcsh:QH301-705.5
Spectroscopy
tRNA Methyltransferases
biology
Organic Chemistry
tRNA methyltransferase
Intron
TRNA Methyltransferase
Thermoplasma acidophilum
RNA
General Medicine
biology.organism_classification
RNA modification
Computer Science Applications
TRNA Methyltransferases
Biochemistry
lcsh:Biology (General)
lcsh:QD1-999
Transfer RNA
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 16
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....625c2073c9d828048c6019c89e021c97