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Phylogenetic analysis of urease-positive thermophilic Campylobacter (UPTC) strains based on the molecular characterization of the flaA gene
- Source :
- Folia microbiologica. 56(5)
- Publication Year :
- 2011
-
Abstract
- Molecular cloning, nucleotide sequencing, and characterization of the flaA gene from additional isolates of urease-positive thermophilic Campylobacter (UPTC) were performed. These isolates were obtained from the natural environment in Northern Ireland (n = 9 from mussels) and in England (n = 1 from sea water). All isolates carried the shorter flaA gene, [open reading frames (ORFs), 1,461 to 1,503 base pairs], without any internal termination codons, and did not carry any flaA pseudogenes. The UPTC isolates were well discriminated by the neighbor joining (NJ) phylogenetic tree constructed based on the putative flaA genes ORFs nucleotide sequence information. In addition, the NJ tree constructed based on the flaA-short variable region sequence information discriminated the Campylobacter lari isolates with a similar degree of discrimination power.
- Subjects :
- Pseudogene
Molecular Sequence Data
Campylobacter lari
Northern Ireland
Microbiology
Polymerase Chain Reaction
Open Reading Frames
Animals
Seawater
Amino Acid Sequence
ORFS
Cloning, Molecular
Neighbor joining
Gene
Phylogeny
Genetics
Phylogenetic tree
biology
Nucleic acid sequence
food and beverages
Campylobacter
General Medicine
Sequence Analysis, DNA
bacterial infections and mycoses
biology.organism_classification
Urease
Recombinant Proteins
Bivalvia
Open reading frame
England
bacteria
Sequence Alignment
Flagellin
Subjects
Details
- ISSN :
- 18749356
- Volume :
- 56
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Folia microbiologica
- Accession number :
- edsair.doi.dedup.....6256c85b1b488425c4cf6fc4655935eb