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Capillary electrophoresis–inductively coupled plasma-mass spectrometry hyphenation for the determination at the nanogram scale of metal affinities and binding constants of phosphorylated ligands
- Source :
- Journal of Chromatography A. 1229:280-287
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- A screening strategy based on hyphenated capillary electrophoresis and inductively coupled plasma mass spectrometry (CE–ICP-MS) was developed to classify phosphorylated ligands according to their europium(III) binding affinity in a hydro-organic medium (sodium formate, pH 3.7, H2O/MeOH 90:10, v/v). Taking advantage of the high sensibility of ICP-MS for detecting phosphorus, this method enabled to assess the affinity of a variety of phosphorylated compounds, including phosphine oxides, thiophosphines, phosphonates, and phosphinates, in less than 1 h and using less than 5 ng of substance. By varying the total europium concentration, complexation constants could be determined according to a sequential multiple run strategy, which proved to be in excellent agreement with the values obtained by UV–Vis absorption spectrophotometric titrations.
- Subjects :
- Formates
chemistry.chemical_element
Ligands
Lanthanoid Series Elements
Biochemistry
Mass Spectrometry
Analytical Chemistry
Metal
chemistry.chemical_compound
Capillary electrophoresis
Europium
Limit of Detection
Inductively coupled plasma mass spectrometry
Principal Component Analysis
Chromatography
Sodium formate
Methanol
Organic Chemistry
Electrophoresis, Capillary
Reproducibility of Results
General Medicine
Phosphorus Compounds
chemistry
visual_art
Linear Models
visual_art.visual_art_medium
Thermodynamics
Spectrophotometry, Ultraviolet
Titration
Absorption (chemistry)
Phosphine
Subjects
Details
- ISSN :
- 00219673
- Volume :
- 1229
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatography A
- Accession number :
- edsair.doi.dedup.....62568987bbaecd7bd051f39b5ab69ff8