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Lectin-like and bacterial-agglutinating activities of heat shock proteins Hsp5 and Hsp90α from amphioxus Branchiostoma japonicum
Lectin-like and bacterial-agglutinating activities of heat shock proteins Hsp5 and Hsp90α from amphioxus Branchiostoma japonicum
- Source :
- Fish & Shellfish Immunology. 95:688-696
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Previous studies have shown that heat shock proteins (Hsps) are broadly associated in immune responses in a variety of animals. However, it remains largely unknown about the direct roles of Hsps during a bacterial infection. In this study, we have cloned and characterized the cDNAs of two Hsp genes in the amphioxus Branchiostoma japonicum, termed Bjhsp5 and Bjhsp90α, the first ones in this evolutionarily important animal. Both Bjhsp5 and Bjhsp90α showed distinct tissue expression patterns, and were inducible by challenge with lipopolysaccharide (LPS) and lipoteichoic acid (LTA), suggesting they may be involved in anti-infectious responses. We also showed that both BjHsp5 and BjHsp90α displayed lectin-like property with affinity to both the Gram-negative and -positive bacteria as well as their signature molecules LPS and LTA, hinting they may both act as a pattern recognition receptor, capable of identifying pathogens. In addition, we found that BjHsp5 and BjHsp90α were both able to agglutinate the Gram-negative and -positive bacteria in the presence of Ca2+, suggesting they may be able to trap the invading pathogens together in vivo, avoiding them moving around and thereby protecting the host from pathogenic attack. These data provide a new angle to the roles of Hsps in immune defense.
- Subjects :
- 0301 basic medicine
Agglutination
Branchiostoma
Lipopolysaccharide
Aquatic Science
03 medical and health sciences
chemistry.chemical_compound
Immune system
Heat shock protein
Animals
Environmental Chemistry
Lectins, C-Type
HSP90 Heat-Shock Proteins
Heat-Shock Proteins
Lancelets
biology
Pattern recognition receptor
Lectin
04 agricultural and veterinary sciences
General Medicine
biology.organism_classification
Immunity, Innate
Cell biology
030104 developmental biology
chemistry
040102 fisheries
biology.protein
0401 agriculture, forestry, and fisheries
Lipoteichoic acid
Bacteria
Subjects
Details
- ISSN :
- 10504648
- Volume :
- 95
- Database :
- OpenAIRE
- Journal :
- Fish & Shellfish Immunology
- Accession number :
- edsair.doi.dedup.....624695db9b235f954f08ac3356d311b3
- Full Text :
- https://doi.org/10.1016/j.fsi.2019.10.074