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Buckwheat FeNramp5 Mediates High Manganese Uptake in Roots
- Source :
- Plant and Cell Physiology. 62:600-609
- Publication Year :
- 2020
- Publisher :
- Oxford University Press (OUP), 2020.
-
Abstract
- Manganese (Mn) is an essential element for plant growth and development, but transporters required for Mn uptake have only been identified in a few plant species. Here, we functionally characterized a member of the natural resistance-associated macrophage proteins (Nramps) family, FeNramp5 in buckwheat (Fagopyrum esculentum Moench), which is known as a species well adapted to acidic soils. FeNramp5 was mainly expressed in the roots, and its expression was upregulated by the deficiency of Mn and Fe. Furthermore, spatial and tissue-specific expression analysis showed that FeNramp5 was expressed in all tissues of the basal root regions. FeNramp5-GFP protein was localized to the plasma membrane when transiently expressed in buckwheat leaf protoplast. FeNramp5 showed the transport activity for Mn2+ and Cd2+ but not for Fe2+ when expressed in yeast. Furthermore, the transport activity for Mn2+ was higher in yeast expressing FeNramp5 than in yeast expressing AtNramp1. FeNramp5 was also able to complement the phenotype of Arabidopsis atnramp1 mutant in terms of the growth and accumulation of Mn and Cd. The absolute expression level of AtNramp1 was comparable to that of FeNramp5 in the roots, but buckwheat accumulated higher Mn than Arabidopsis when grown under the same condition. Further analysis showed that at least motif B in FeNramp5 seems important for its high transport activity for Mn. These results indicate that FeNramp5 is a transporter for the uptake of Mn and Cd and its higher transport activity for Mn is probably associated with higher Mn accumulation in buckwheat.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Physiology
Amino Acid Motifs
Mutant
Arabidopsis
chemistry.chemical_element
Plant Science
Manganese
Plant Roots
01 natural sciences
03 medical and health sciences
Gene Expression Regulation, Plant
Yeasts
Soil pH
Cation Transport Proteins
Phylogeny
Plant Proteins
biology
Arabidopsis Proteins
Chemistry
Protoplasts
Biological Transport
Transporter
Cell Biology
General Medicine
Protoplast
Plants, Genetically Modified
biology.organism_classification
Phenotype
Yeast
030104 developmental biology
Biochemistry
Metals
Mutation
Fagopyrum
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14719053 and 00320781
- Volume :
- 62
- Database :
- OpenAIRE
- Journal :
- Plant and Cell Physiology
- Accession number :
- edsair.doi.dedup.....623dc7a3b9b43ce375607f837495d6ee