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Carboxyl formation from methyl via triple hydroxylations by XiaM in xiamycin A biosynthesis
- Source :
- Organic letters. 14(24)
- Publication Year :
- 2012
-
Abstract
- The P450 enzyme XiaM was identified as a candidate to form the C-24 carboxyl group in xiamycin A (1). Alteration of medium composition led to the discovery of four new compounds from the ΔxiaM and the ΔxiaK (encoding an aromatic ring hydroxylase) mutants. Biotransformation experiments revealed that XiaM was capable of converting a methyl group to a carboxyl group through diol and aldehyde intermediates.
- Subjects :
- chemistry.chemical_classification
Stereochemistry
Organic Chemistry
Diol
Oxidoreductases, N-Demethylating
Hydroxylation
Biochemistry
Aldehyde
Aryl Hydrocarbon Hydroxylases
Streptomyces
Mixed Function Oxygenases
chemistry.chemical_compound
Cytochrome P-450 CYP2B6
Enzyme
chemistry
Biosynthesis
Biotransformation
Cytochrome P-450 Enzyme System
Escherichia coli
Physical and Theoretical Chemistry
Sesquiterpenes
Methyl group
Subjects
Details
- ISSN :
- 15237052
- Volume :
- 14
- Issue :
- 24
- Database :
- OpenAIRE
- Journal :
- Organic letters
- Accession number :
- edsair.doi.dedup.....62352463ac5686a1d0c22e42e5bdad70