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Nanopore sequencing in non-human forensic genetics
- Source :
- Ogden, R, Vasiljevic, N & Prost, S 2021, ' Nanopore sequencing in non-human forensic genetics ', Emerging Topics in Life Sciences . https://doi.org/10.1042/ETLS20200287, Emerging Topics in Life Sciences
- Publication Year :
- 2021
- Publisher :
- Portland Press Ltd., 2021.
-
Abstract
- The past decade has seen a rapid expansion of non-human forensic genetics coinciding with the development of 2nd and 3rd generation DNA sequencing technologies. Nanopore sequencing is one such technology that offers massively parallel sequencing at a fraction of the capital cost of other sequencing platforms. The application of nanopore sequencing to species identification has already been widely demonstrated in biomonitoring studies and has significant potential for non-human forensic casework, particularly in the area of wildlife forensics. This review examines nanopore sequencing technology and assesses its potential applications, advantages and drawbacks for use in non-human forensics, alongside other next-generation sequencing platforms and as a possible replacement to Sanger sequencing. We assess the specific challenges of sequence error rate and the standardisation of consensus sequence production, before discussing recent progress in the validation of nanopore sequencing for use in forensic casework. We conclude that nanopore sequencing may be able to play a considerable role in the future of non-human forensic genetics, especially for applications to wildlife law enforcement within emerging forensic laboratories.
- Subjects :
- Forensic Genetics
0106 biological sciences
Bioinformatics
species identification
MinION
1100 General Agricultural and Biological Sciences
2700 General Medicine
Computational biology
010603 evolutionary biology
01 natural sciences
General Biochemistry, Genetics and Molecular Biology
DNA sequencing
10127 Institute of Evolutionary Biology and Environmental Studies
03 medical and health sciences
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Zoology & Marine Biology
1300 General Biochemistry, Genetics and Molecular Biology
wildlife forensics
Species identification
Review Articles
Diagnostics & Biomarkers
030304 developmental biology
Sanger sequencing
0303 health sciences
Massive parallel sequencing
Rapid expansion
High-Throughput Nucleotide Sequencing
Sequence Analysis, DNA
minion
10218 Institute of Legal Medicine
Nanopore Sequencing
Minion
symbols
570 Life sciences
biology
590 Animals (Zoology)
Nanopore sequencing
General Agricultural and Biological Sciences
Forensic genetics
Subjects
Details
- ISSN :
- 23978562 and 23978554
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Emerging Topics in Life Sciences
- Accession number :
- edsair.doi.dedup.....6222039a31aaab7475f9cf5504300d2d