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Identifying toxic fractions of wheat gluten and their effect on the jejunal mucosa in coeliac disease
- Source :
- Gut. 15:931-946
- Publication Year :
- 1974
- Publisher :
- BMJ, 1974.
-
Abstract
- The toxicity of three fractions (A, B, and C) obtained by ultrafiltration of a peptic: tryptic digest of gluten has been assessed by serial feeding experiments in patients with treated coeliac disease. The first fraction (A), which contains amino acids and oligopeptides, produced no damage to the jejunal mucosa. The other two fractions (B and C) both caused mucosal damage. Fraction B, which contains the products of digestion of smaller molecular weight, consists of polypeptides which are concentrated in the region of 8000 molecular weight. It contains no gliadin (molecular weight 50 000) or gluten. Ultrastructural evidence of damage was visible six hours after challenge with fraction B and by 10 hours histological abnormalities were also present. Ultrastructural abnormalities occurred early in the epithelial cells and preceded changes in the basement membrane and capillaries. The disaccharidases showed a pronounced depression in all three subjects by 24 hours. The rapid onset of damage after challenge, coupled with the evidence of recovery as soon as 72 hours later, is more in keeping with a direct action on the surface epithelial cells rather than an immune mechanism.
- Subjects :
- Male
Glutens
Chromatography, Paper
Nitrogen
Ultrafiltration
Biology
Disaccharidases
digestive system
Coeliac disease
Jejunum
Intestinal mucosa
medicine
Humans
Amino Acids
Intestinal Mucosa
Triticum
chemistry.chemical_classification
Gastroenterology
nutritional and metabolic diseases
Articles
medicine.disease
Molecular biology
Gluten
digestive system diseases
Disaccharidase
Mitochondria
Molecular Weight
Celiac Disease
Microscopy, Electron
medicine.anatomical_structure
Biochemistry
chemistry
Toxicity
biology.protein
Electrophoresis, Polyacrylamide Gel
Female
Digestion
Gliadin
Subjects
Details
- ISSN :
- 00175749
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Gut
- Accession number :
- edsair.doi.dedup.....621d965e13aaeea2b5bf6a0307aa0b6b