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Androgen regulation and site specificity of angiotensinogen gene expression and secretion in rat adipocytes

Authors :
Valérie Serazin-Leroy
Yves Giudicelli
Philippe de Mazancourt
Mireille Morot
Source :
American Journal of Physiology-Endocrinology and Metabolism. 279:E1398-E1405
Publication Year :
2000
Publisher :
American Physiological Society, 2000.

Abstract

Adipose tissue is an important source of angiotensinogen (ATG), and hypertension is commonly associated with android obesity. Therefore, we tested the hypothesis that androgens may control ATG gene expression and secretion in rat fat cells. In intact male rats, ATG mRNA expression (Northern blot and co-reverse transcription-polymerase chain reaction analysis) and protein secretion were significantly higher in deep intra-abdominal (perirenal and epididymal) than in subcutaneous adipocytes. After castration, ATG mRNA was reduced almost 50% in the three fat deposits, with parallel changes in ATG protein secretion. Conversely, testosterone treatment fully restored the ATG mRNA decrease after castration, whatever the anatomical origin of the adipocytes. Finally, a 24-h in vitro exposure of perirenal fat cells or differentiated preadipocytes from castrated rats to testosterone or dihydrotestosterone (10 nM free hormone concentration) increased ATG mRNA expression by 50–100%, an effect that was prevented by the anti-androgen cyproterone acetate. These data, demonstrating both in vivo and in vitro androgen induction of ATG mRNA expression in rat adipocytes, add further weight to the hypothesis of a link between adipose tissue ATG production, androgens, and android obesity-related hypertension.

Details

ISSN :
15221555 and 01931849
Volume :
279
Database :
OpenAIRE
Journal :
American Journal of Physiology-Endocrinology and Metabolism
Accession number :
edsair.doi.dedup.....620e02c0a1262ad70c6bf2aaf46e81bd