Back to Search
Start Over
Androgen regulation and site specificity of angiotensinogen gene expression and secretion in rat adipocytes
- Source :
- American Journal of Physiology-Endocrinology and Metabolism. 279:E1398-E1405
- Publication Year :
- 2000
- Publisher :
- American Physiological Society, 2000.
-
Abstract
- Adipose tissue is an important source of angiotensinogen (ATG), and hypertension is commonly associated with android obesity. Therefore, we tested the hypothesis that androgens may control ATG gene expression and secretion in rat fat cells. In intact male rats, ATG mRNA expression (Northern blot and co-reverse transcription-polymerase chain reaction analysis) and protein secretion were significantly higher in deep intra-abdominal (perirenal and epididymal) than in subcutaneous adipocytes. After castration, ATG mRNA was reduced almost 50% in the three fat deposits, with parallel changes in ATG protein secretion. Conversely, testosterone treatment fully restored the ATG mRNA decrease after castration, whatever the anatomical origin of the adipocytes. Finally, a 24-h in vitro exposure of perirenal fat cells or differentiated preadipocytes from castrated rats to testosterone or dihydrotestosterone (10 nM free hormone concentration) increased ATG mRNA expression by 50–100%, an effect that was prevented by the anti-androgen cyproterone acetate. These data, demonstrating both in vivo and in vitro androgen induction of ATG mRNA expression in rat adipocytes, add further weight to the hypothesis of a link between adipose tissue ATG production, androgens, and android obesity-related hypertension.
- Subjects :
- Male
medicine.medical_specialty
Physiology
medicine.drug_class
Endocrinology, Diabetes and Metabolism
Prohormone
Angiotensinogen
Gene Expression
Adipose tissue
Biology
Dexamethasone
Rats, Sprague-Dawley
chemistry.chemical_compound
Physiology (medical)
Internal medicine
Adipocyte
Gene expression
Renin–angiotensin system
Adipocytes
medicine
Animals
Testosterone
Secretion
RNA, Messenger
Cyproterone Acetate
Gonadal Steroid Hormones
Glucocorticoids
Cells, Cultured
Reverse Transcriptase Polymerase Chain Reaction
Androgen Antagonists
Dihydrotestosterone
Blotting, Northern
Androgen
Rats
Endocrinology
chemistry
Orchiectomy
medicine.drug
Subjects
Details
- ISSN :
- 15221555 and 01931849
- Volume :
- 279
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Endocrinology and Metabolism
- Accession number :
- edsair.doi.dedup.....620e02c0a1262ad70c6bf2aaf46e81bd