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Localization of arginine vasopressin-neurophysin II messenger ribonucleic acid in the hypothalamus of control and Brattleboro rats by hybridization histochemistry with a synthetic pentadecamer oligonucleotide probe
- Source :
- Endocrinology. 116(6)
- Publication Year :
- 1985
-
Abstract
- By using 32P-labeled oligonucleotide probes for in situ DNA-RNA hybridization, we have defined the distribution of messenger RNA (MRNA) for arginine vasopressin-neurophysin II (AVP-NPII) in the rat hypothalamus. With a specific pentadecamer nucleotide probe, strong specific hybridization is seen in supraoptic, suprachiasmatic, and paraventricular nuclei in both Sprague-Dawley and Brattleboro rats. This finding, of equivalent distribution of AVP-NPII mRNA in Brattleboro and control hypothalami, confirms and extends the recent report of equivalent levels of AVP-NPII mRNA by Northern blot analysis. (Endocrinology 116: 2366–2368, 1985)
- Subjects :
- Male
endocrine system
medicine.medical_specialty
Vasopressin
Arginine
Hypothalamus
Oligonucleotides
Neurophysins
Biology
Endocrinology
Internal medicine
medicine
Animals
Northern blot
RNA, Messenger
Messenger RNA
Oligonucleotide
Histocytochemistry
Nucleic Acid Hybridization
Rats, Brattleboro
Rats, Inbred Strains
Molecular biology
Rats
Arginine Vasopressin
nervous system
Biochemistry
Oligomer restriction
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- ISSN :
- 00137227
- Volume :
- 116
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Endocrinology
- Accession number :
- edsair.doi.dedup.....61e8464948a53e79052be08eb9867cce