Supplementary Figures 1 - 10 from Real-time In Vivo Molecular Detection of Primary Tumors and Metastases with Ratiometric Activatable Cell-Penetrating Peptides

PDF file - 835K, Supplementary Fig 1. Structures of RACPP1,2,3 and uncleavable control. Supplementary Fig 2. Structures of synthesized RACPPs with varying acceptor fluorophores. Supplementary Fig 3. Characterization of RACPP2. Supplementary Fig 4. Ratiometric RACPP images show superior contrast compared to Cy5 fluorescence alone. Supplementary Fig 5. RACPPs show specificity for MMP2,9 cleavage in transgenic animal models. Supplementary Fig 6. RACPPs show tumor specific cleavage and uptake. Supplementary Fig 7. Non-ratiometric ACPP has poor contrast in liver metastases. Supplementary Fig 8. Ratiometric analyses using RACPPs show sensitive detection of metastatic lymph nodes. Supplementary Fig 9. LCMS characterization of RACPPs. Supplementary Fig 10. Synthetic schemes for RACPPs.