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Lack of an effect of collecting duct-specific deletion of adenylyl cyclase 3 on renal Na+and water excretion or arterial pressure
- Source :
- American Journal of Physiology-Renal Physiology. 306:F597-F607
- Publication Year :
- 2014
- Publisher :
- American Physiological Society, 2014.
-
Abstract
- cAMP is a key mediator of connecting tubule and collecting duct (CD) Na+and water reabsorption. Studies performed in vitro have suggested that CD adenylyl cyclase (AC)3 partly mediates the actions of vasopressin; however, the physiological role of CD AC3 has not been determined. To assess this, mice were developed with CD-specific disruption of AC3 [CD AC3 knockout (KO)]. Inner medullary CDs from these mice exhibited 100% target gene recombination and had reduced ANG II- but not vasopressin-induced cAMP accumulation. However, there were no differences in urine volume, urinary urea excretion, or urine osmolality between KO and control mice during normal water intake or varying degrees of water restriction in the presence or absence of chronic vasopressin administration. There were no differences between CD AC3 KO and control mice in arterial pressure or urinary Na+or K+excretion during a normal or high-salt diet, whereas plasma renin and vasopressin concentrations were similar between the two genotypes. Patch-clamp analysis of split-open cortical CDs revealed no difference in epithelial Na+channel activity in the presence or absence of vasopressin. Compensatory changes in AC6 were not responsible for the lack of a renal phenotype in CD AC3 KO mice since combined CD AC3/AC6 KO mice had similar arterial pressure and renal Na+and water handling compared with CD AC6 KO mice. In summary, these data do not support a significant role for CD AC3 in the regulation of renal Na+and water excretion in general or vasopressin regulation of CD function in particular.
- Subjects :
- Male
medicine.medical_specialty
Physiology
Sodium
Diuresis
chemistry.chemical_element
Blood Pressure
Adenylyl cyclase
Mice
chemistry.chemical_compound
Mediator
Internal medicine
medicine
Animals
Kidney Tubules, Collecting
Sodium Chloride, Dietary
Mice, Knockout
Chemistry
Reabsorption
Articles
Connecting tubule
In vitro
medicine.anatomical_structure
Endocrinology
Female
Duct (anatomy)
Adenylyl Cyclases
Subjects
Details
- ISSN :
- 15221466 and 1931857X
- Volume :
- 306
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Renal Physiology
- Accession number :
- edsair.doi.dedup.....61d75cb72355f233b5be9635a3d87327