Back to Search
Start Over
Anti-angiogenesis therapy based on the bone marrow-derived stromal cells genetically engineered to express sFlt-1 in mouse tumor model
- Source :
- BMC Cancer, BMC Cancer, Vol 8, Iss 1, p 306 (2008)
- Publication Year :
- 2008
- Publisher :
- Springer Science and Business Media LLC, 2008.
-
Abstract
- Background Bone marrow-derived stromal cells (BMSCs) are important for development, tissue cell replenishment, and wound healing in physiological and pathological conditions. BMSCs were found to preferably reach sites undergoing the process of cell proliferation, such as wound and tumor, suggesting that BMSCs may be used as a vehicle for gene therapy of tumor. Methods Mouse BMSCs were loaded with recombinant adenoviruses which express soluble Vascular Endothelial Growth Factor Receptor-1 (sFlt-1). The anti-angiogenesis of sFlt-1 in BMSCs was determined using endothelial cells proliferation inhibition assay and alginate encapsulation assay. The anti-tumor effects of BMSCs expressing sFlt-1 through tail-vein infusion were evaluated in two mouse tumor metastases models. Results BMSCs genetically modified with Adv-GFP-sFlt-1 could effectively express and secret sFlt-1. BMSCs loaded with sFlt-1 gene could preferentially home to tumor loci and decrease lung metastases and prolong lifespan in mouse tumor model through inducing anti-angiogenesis and apoptosis in tumors. Conclusion We demonstrated that BMSCs might be employed as a promising vehicle for tumor gene therapy which can effectively not only improve the concentration of anticancer therapeutics in tumors, but also modify the tumor microenvironment.
- Subjects :
- Male
Cancer Research
Pathology
medicine.medical_specialty
Stromal cell
Genetic enhancement
Apoptosis
Bone Marrow Cells
Transfection
lcsh:RC254-282
Adenoviridae
Mice
chemistry.chemical_compound
stomatognathic system
Cell Line, Tumor
In Situ Nick-End Labeling
Genetics
medicine
Animals
Neoplasm Metastasis
Cell Proliferation
Mice, Inbred BALB C
Tumor microenvironment
Vascular Endothelial Growth Factor Receptor-1
Neovascularization, Pathologic
business.industry
hemic and immune systems
Genetic Therapy
Neoplasms, Experimental
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Mice, Inbred C57BL
Vascular endothelial growth factor
Disease Models, Animal
medicine.anatomical_structure
Oncology
chemistry
embryonic structures
Cancer research
Female
Bone marrow
Stromal Cells
Stem cell
business
Wound healing
Research Article
Subjects
Details
- ISSN :
- 14712407
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- BMC Cancer
- Accession number :
- edsair.doi.dedup.....61c8375a8539677bd60f0b1c4d15f78f