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Usefulness of the Ultrasensitive Anti-Müllerian Hormone Assay for Predicting True Ovarian Reserve
- Source :
- Reproductive Sciences. 23:756-760
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- Serum concentration of anti-Müllerian hormone (AMH) is a useful marker for ovarian reserve. Measurement of AMH in clinical practice has gained widespread use to predict parameters such as the ovarian response, menopause, and recovery after chemotherapy. However, undetectable AMH levels assayed by conventional enzyme-linked immunosorbent assay (ELISA) kits fail to predict depletion of follicles because of low sensitivity of the kits. We investigated whether a recently developed ultrasensitive ELISA kit, picoAMH, would be more effective at detecting very low AMH levels in association with menstrual status. We analyzed 68 women with undetectable serum AMH levels using an ELISA kit, AMH Gen II. The AMH concentration of the same samples was detected in 36 samples using picoAMH; 32 samples were within the standard range, and 4 samples were out of the standard range but still detectable. Thirty-two women whose AMH levels were undetectable using the picoAMH all showed amenorrhea. We also found a significant correlation between the classes of serum AMH levels (undetectable, detectable under the limit of quantification, and measurable within the assay range) and menstrual status. Five of the 6 amenorrheic women with detectable AMH eventually achieved follicle growth. The present study demonstrated that very low AMH levels detectable using picoAMH correspond well to current and future ovulation status. This suggests that serum AMH levels can be useful for the assessment of ovarian reserve and follow-up of women with a declined ovarian reserve.
- Subjects :
- Adult
Anti-Mullerian Hormone
0301 basic medicine
endocrine system
medicine.medical_specialty
endocrine system diseases
Enzyme-Linked Immunosorbent Assay
Ovulation status
03 medical and health sciences
Elisa kit
0302 clinical medicine
Internal medicine
medicine
Humans
Ovarian Reserve
Ovarian reserve
Amenorrhea
Menstrual Cycle
Follicle growth
030219 obstetrics & reproductive medicine
biology
urogenital system
business.industry
Obstetrics and Gynecology
Anti-Müllerian hormone
medicine.disease
female genital diseases and pregnancy complications
Oligomenorrhea
Menopause
030104 developmental biology
Endocrinology
biology.protein
Female
Follicle Stimulating Hormone
medicine.symptom
business
hormones, hormone substitutes, and hormone antagonists
Hormone
Subjects
Details
- ISSN :
- 19337205 and 19337191
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Reproductive Sciences
- Accession number :
- edsair.doi.dedup.....61c39160b149db800cfa9fb6b0d42e92