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Sensitive detection of p53 tumor suppressor gene using an enzyme-based solid-state electrochemiluminescence sensing platform
- Source :
- Biosensorsbioelectronics. 26(8)
- Publication Year :
- 2010
-
Abstract
- An enzyme-based solid-state electrochemiluminescence (ECL) sensing platform for sensitive detection of a single point mutation is developed successfully using p53 tumor suppressor gene as a model analyte. A composite of multiwalled carbon nanotubes and Ruthenium (II) tris-(bipyridine) (MWNTs–Ru(bpy)32+) was prepared and coated on an electrode surface, which was covered by polypyrrole (PPy) to immobilize ssDNA. Then, the ssDNA recognized the gold nanoparticle (AuNP)-labeled p53 tumor suppressor gene, and produced AuNP-dsDNA electrode with AuNP layer. The surface adsorbed the glucose-dehydrogenase (GDH) molecules for producing ECL signal. This system combined enzyme reaction with ECL detection, and it can recognize sequence-specific wild type p53 sequence (wtp53) and muted type p53 sequence (mtp53) with discrimination of up to 56.3%. The analytic results were sensitive and specific. It holds promise for the diagnosis and management of cancer.
- Subjects :
- Analyte
Chemistry
Nanotubes, Carbon
Biomedical Engineering
Biophysics
Analytical chemistry
Nanoparticle
chemistry.chemical_element
Reproducibility of Results
General Medicine
Biosensing Techniques
Polypyrrole
Genes, p53
Combinatorial chemistry
Ruthenium
Bipyridine
chemistry.chemical_compound
Glucose dehydrogenase
Electrode
Calibration
Luminescent Measurements
Electrochemistry
Electrochemiluminescence
Electrodes
Biotechnology
Subjects
Details
- ISSN :
- 18734235
- Volume :
- 26
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Biosensorsbioelectronics
- Accession number :
- edsair.doi.dedup.....61ba614e9e6587df9263b198bda34beb