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Immune Responses Induced by Gene Gun or Intramuscular Injection of DNA Vaccines That Express Immunogenic Regions of the Serine Repeat Antigen from Plasmodium falciparum
- Source :
- Infection and Immunity. 67:5163-5169
- Publication Year :
- 1999
- Publisher :
- American Society for Microbiology, 1999.
-
Abstract
- The liver- and blood-stage-expressed serine repeat antigen (SERA) of Plasmodium falciparum is a candidate protein for a human malaria vaccine. We compared the immune responses induced in mice immunized with SERA-expressing plasmid DNA vaccines delivered by intramuscular (i.m.) injection or delivered intradermally by Gene Gun immunization. Mice were immunized with a pcdna3 plasmid encoding the entire 47-kDa domain of SERA (amino acids 17 to 382) or the N-terminal domain (amino acids 17 to 110) of SERA. Minimal antibody responses were detected following DNA vaccination with the N-terminal domain of SERA, suggesting that the N-terminal domain alone is not highly immunogenic by this route of vaccine delivery. Immunization of mice by Gene Gun delivery of the 47-kDa domain of SERA elicited a significantly higher serum antibody titer to the antigen than immunization of mice by i.m. injection with the same plasmid did. The predominant isotype subclass of the antibodies elicited to the SERA protein following i.m. and Gene Gun immunizations with SERA plasmid DNA was immunoglobulin G1. Coimmunization of mice with SERA plasmid DNA and a plasmid expressing the hepatitis B surface antigen (pCMV-s) by the i.m. route resulted in higher anti-SERA titers than those generated in mice immunized with the SERA DNA plasmid alone. Vaccination with DNA may provide a viable alternative or may be used in conjunction with protein-based subunit vaccines to maximize the efficacy of a human malaria vaccine that includes immunogenic regions of the SERA protein.
- Subjects :
- Repetitive Sequences, Amino Acid
Plasmodium falciparum
Immunology
Antibodies, Protozoan
Antigens, Protozoan
Biology
Injections, Intramuscular
Microbiology
DNA vaccination
Gene gun
Mice
Plasmid
Antigen
Malaria Vaccines
Vaccines, DNA
Animals
Mice, Inbred BALB C
Malaria vaccine
Immunogenicity
Biolistics
Isotype
Virology
Immunoglobulin Isotypes
Infectious Diseases
biology.protein
Female
Parasitology
Fungal and Parasitic Infections
Antibody
Subjects
Details
- ISSN :
- 10985522 and 00199567
- Volume :
- 67
- Database :
- OpenAIRE
- Journal :
- Infection and Immunity
- Accession number :
- edsair.doi.dedup.....61b7776302970a66277bb09c87a10c3a