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Relaxin has beneficial effects on liver lipidome and metabolic enzymes
- Source :
- The FASEB Journal. 35
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Relaxin is an insulin-like hormone with pleiotropic protective effects in several organs, including the liver. We aimed to characterize its role in the control of hepatic metabolism in healthy rats. Sprague-Dawley rats were treated with human recombinant relaxin-2 for 2 weeks. The hepatic metabolic profile was analyzed using UHPLC-MS platforms. Hepatic gene expression of key enzymes of desaturation (Fads1/Fads2) of n-6 and n-3 polyunsaturated fatty acids (PUFAs), of phosphatidylethanolamine (PE) N-methyltransferase (Pemt), of fatty acid translocase Cd36, and of glucose-6-phosphate isomerase (Gpi) were quantified by Real Time-PCR. Activation of 5'AMP-activated protein kinase (AMPK) was analyzed by Western Blot. Relaxin-2 significantly modified the hepatic levels of 19 glycerophospholipids, 2 saturated (SFA) and 1 monounsaturated (MUFA) fatty acids (FA), 3 diglycerides, 1 sphingomyelin, 2 aminoacids, 5 nucleosides, 2 nucleotides, 1 carboxylic acid, 1 redox electron carrier, and 1 vitamin. The most noteworthy changes corresponded to the substantially decreased lysoglycerophospholipids, and to the clearly increased FA (16:1n-7/16:0) and MUFA + PUFA/SFA ratios, suggesting enhanced desaturase activity. Hepatic gene expression of Fads1, Fads2, and Pemt, which mediates lipid balance and liver health, was increased by relaxin-2, while mRNA levels of the main regulator of hepatic FA uptake Cd36, and of the essential glycolysis enzyme Gpi, were decreased. Relaxin-2 augmented the hepatic activation of the hepatoprotector and master regulator of energy homeostasis AMPK. Relaxin-2 treatment also rised FADS1, FADS2, and PEMT gene expression in cultured Hep G2 cells. Our results bring to light the hepatic metabolic features stimulated by relaxin, a promising hepatoprotective molecule.
- Subjects :
- Fatty Acid Desaturases
Male
0301 basic medicine
medicine.medical_specialty
FADS1
Phosphatidylethanolamine N-Methyltransferase
CD36
FADS2
Glycerophospholipids
Biochemistry
Fatty Acids, Monounsaturated
Rats, Sprague-Dawley
03 medical and health sciences
Delta-5 Fatty Acid Desaturase
0302 clinical medicine
Cell Line, Tumor
Internal medicine
Fatty Acids, Omega-3
Genetics
medicine
Animals
Homeostasis
Humans
Glycolysis
Molecular Biology
Relaxin
chemistry.chemical_classification
biology
Phosphatidylethanolamines
Fatty Acids
AMPK
Hep G2 Cells
Recombinant Proteins
Rats
030104 developmental biology
Endocrinology
Enzyme
Liver
chemistry
Lipidomics
Metabolome
biology.protein
030217 neurology & neurosurgery
Biotechnology
Polyunsaturated fatty acid
Subjects
Details
- ISSN :
- 15306860 and 08926638
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- The FASEB Journal
- Accession number :
- edsair.doi.dedup.....61a0501825f57a9765626258e5d4e6a6