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Imaging of Cell Structures Using Optimized Soft X-ray Contact Microscopy
- Source :
- Applied Sciences, Volume 10, Issue 19, Applied Sciences, Vol 10, Iss 6895, p 6895 (2020)
- Publication Year :
- 2020
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2020.
-
Abstract
- This work is to study the relationship between the exposure conditions and the quality of cell imaging with soft X-ray contact microscopy (SXCM). It is a crucial step in the efficient visualization of cell structures. Three different human cell lines: DU145 prostate carcinoma cells, HCC38 breast cancer cells, and Poietics mesenchymal stem cells were used to establish the optimal exposure conditions in SXCM. The image quality depended on the soft X-ray (SXR) absorbed energy and photoresist development conditions. At lower SXR energy (200 or 400 SXR pulses), sharp cell edges, membrane projections, and cell&ndash<br />cell connections were visible. In contrast, higher energy (600 or 800 SXR pulses) allowed observation of the cytoskeleton and the nucleus in a cell type-dependent manner (the influence of cell thickness and internal complexity was noted).
- Subjects :
- Materials science
Cell
02 engineering and technology
Photoresist
lcsh:Technology
lcsh:Chemistry
03 medical and health sciences
DU145
Microscopy
medicine
General Materials Science
Cytoskeleton
cellular structure
Instrumentation
lcsh:QH301-705.5
030304 developmental biology
Fluid Flow and Transfer Processes
0303 health sciences
lcsh:T
Process Chemistry and Technology
Mesenchymal stem cell
General Engineering
soft X-ray contact microscopy
high-resolution imaging
021001 nanoscience & nanotechnology
lcsh:QC1-999
Computer Science Applications
Membrane
medicine.anatomical_structure
lcsh:Biology (General)
lcsh:QD1-999
lcsh:TA1-2040
Biophysics
0210 nano-technology
lcsh:Engineering (General). Civil engineering (General)
Nucleus
lcsh:Physics
Subjects
Details
- Language :
- English
- ISSN :
- 20763417
- Database :
- OpenAIRE
- Journal :
- Applied Sciences
- Accession number :
- edsair.doi.dedup.....619ac9752e74d4965fe15c394b2e858f
- Full Text :
- https://doi.org/10.3390/app10196895