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Deoxynivalenol and Zearalenone: Different Mycotoxins with Different Toxic Effects in the Sertoli Cells of Equus asinus
- Source :
- Cells, Cells, Vol 10, Iss 1898, p 1898 (2021), Volume 10, Issue 8
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- (1) Background: Deoxynivalenol (DON) and zearalenone (ZEA) are type B trichothecene mycotoxins that exert serious toxic effects on the reproduction of domestic animals. However, there is little information about the toxicity of mycotoxins on testis development in Equus asinus. This study investigated the biological effects of DON and ZEA exposure on Sertoli cells (SCs) of Equus asinus<br />(2) Methods: We administered 10 μM and 30 μM DON and ZEA to cells cultured in vitro<br />(3) Results: The results showed that 10 μM DON exposure remarkably changed pyroptosis-associated genes and that 30 μM ZEA exposure changed inflammation-associated genes in SCs. The mRNA expression of cancer-promoting genes was remarkably upregulated in the cells exposed to DON or 30 μM ZEA<br />in particular, DON and ZEA remarkably disturbed the expression of androgen and oestrogen secretion-related genes. Furthermore, quantitative RT-PCR, Western blot, and immunofluorescence analyses verified the different expression patterns of related genes in DON- and ZEA-exposed SCs<br />(4) Conclusions: Collectively, these results illustrated the impact of exposure to different toxins and concrete toxicity on the mRNA expression of SCs from Equus asinus in vitro.
- Subjects :
- Male
0301 basic medicine
endocrine system
QH301-705.5
Trichothecene
Apoptosis
Biology
Article
Equus asinus
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Western blot
medicine
Animals
Gene Regulatory Networks
RNA, Messenger
Biology (General)
Mycotoxin
Zearalenone
DON
Cells, Cultured
Sertoli Cells
medicine.diagnostic_test
pyroptosis
ZEA
food and beverages
Equidae
General Medicine
Sertoli cell
biology.organism_classification
Molecular biology
In vitro
030104 developmental biology
medicine.anatomical_structure
Gene Expression Regulation
chemistry
030220 oncology & carcinogenesis
Toxicity
RNA-seq
Transcriptome
Trichothecenes
Subjects
Details
- ISSN :
- 20734409
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Cells
- Accession number :
- edsair.doi.dedup.....617a84c6da6d288c3f086c3471a3c52f