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Negative regulation of geminin by CDK-dependent ubiquitination controls replication licensing
- Source :
- Europe PubMed Central, Scopus-Elsevier
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Abstract
- The replication licensing system ensures the precise duplication of chromosomal DNA in each cell cycle. In metazoans, a small protein called geminin plays a central role in negatively regulating licensing late in the cell cycle. Recent work using Xenopus egg extracts shows how geminin activity is downregulated on exit from metaphase in a process that requires mitotic cyclin-dependent kinases (CDKs). Geminin is polyubiquitinated by the Anaphase Promoting Complex, but instead of being proteolysed - the normal fate of polyubiquitinated proteins - much of the geminin is deubiquitinated, leaving it inactive. These results suggest a simple model for how precise chromosome duplication is ensured in the Xenopus model system.
Details
- Database :
- OpenAIRE
- Journal :
- Europe PubMed Central, Scopus-Elsevier
- Accession number :
- edsair.doi.dedup.....617724b72f87c2209e5543bf0fe7d7a7