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Characterization of the complete mitochondrial genome of the cloacal tapeworm Cloacotaenia megalops (Cestoda: Hymenolepididae)
- Source :
- Parasites & Vectors
- Publisher :
- Springer Nature
-
Abstract
- Background The cloacal tapeworm Cloacotaenia megalops (Hymenolepididae) is one of the most common cestode parasites of domestic and wild ducks worldwide. However, limited information is available regarding its epidemiology, biology, genetics and systematics. This study provides characterisation of the complete mitochondrial (mt) genome of C. megalops. Methods The complete mt genome of C. megalops was obtained by long PCR, sequenced and annotated. Results The length of the entire mt genome of C. megalops is 13,887 bp; it contains 12 protein-coding, 2 ribosomal RNA and 22 transfer RNA genes, but lacks an atp8 gene. The mt gene arrangement of C. megalops is identical to that observed in Anoplocephala magna and A. perfoliata (Anoplocephalidae), Dipylidium caninum (Dipylidiidae) and Hymenolepis diminuta (Hymenolepididae), but differs from that reported in taeniids owing to the position shift between the tRNA (L1) and tRNA (S2) genes. The phylogenetic position of C. megalops was inferred using Maximum likelihood and Bayesian inference methods based on the concatenated amino acid data for 12 protein-coding genes. Phylogenetic trees showed that C. megalops is sister to Anoplocephala spp. (Anoplocephalidae) + Pseudanoplocephala crawfordi + Hymenolepis spp. (Hymenolepididae) indicating that the family Hymenolepididae is paraphyletic. Conclusions The complete mt genome of C. megalops is sequenced. Phylogenetic analyses provided an insight into the phylogenetic relationships among the families Anoplocephalidae, Hymenolepididae, Dipylidiidae and Taeniidae. This novel genomic information also provides the opportunity to develop useful genetic markers for studying the molecular epidemiology, biology, genetics and systematics of C. megalops. Electronic supplementary material The online version of this article (doi:10.1186/s13071-016-1782-0) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
Systematics
food.ingredient
Anoplocephalidae
Short Report
Zoology
Biology
DNA, Mitochondrial
Polymerase Chain Reaction
Genome
03 medical and health sciences
food
Mitochondrial genome
Cloaca
Gene Order
Animals
Hymenolepididae
Phylogenetic analyses
Phylogeny
Poultry Diseases
Genome, Helminth
Likelihood Functions
Cloacotaenia megalops
Phylogenetic tree
Anoplocephala
Tapeworm
Bayes Theorem
Sequence Analysis, DNA
Ribosomal RNA
Cestode Infections
biology.organism_classification
Ducks
Genes, Mitochondrial
030104 developmental biology
Infectious Diseases
RNA, Ribosomal
Evolutionary biology
Genome, Mitochondrial
Taeniidae
Cestoda
Parasitology
Subjects
Details
- Language :
- English
- ISSN :
- 17563305
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Parasites & Vectors
- Accession number :
- edsair.doi.dedup.....617038a1ea06bdfbaf42d26b2ce2c4a8
- Full Text :
- https://doi.org/10.1186/s13071-016-1782-0