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CD81 and CD9 work independently as extracellular components upon fusion of sperm and oocyte
- Source :
- Biology Open, Biology Open, Vol 1, Iss 7, Pp 640-647 (2012)
- Publication Year :
- 2012
-
Abstract
- Summary When a sperm and oocyte unite into one cell upon fertilization, membranous fusion between the sperm and oocyte occurs. In mice, Izumo1 and a tetraspanin molecule CD9 are required for sperm-oocyte fusion as one of the oocyte factors, and another tetraspanin molecule CD81 is also thought to involve in this process. Since these two tetraspanins often form a complex upon cell-cell interaction, it is probable that such a complex is also formed in sperm-oocyte interaction; however, this possibility is still under debate among researchers. Here we assessed this problem using mouse oocytes. Immunocytochemical analysis demonstrated that both CD9 and CD81 were widely distributed outside the oocyte cell membrane, but these molecules were separate, forming bilayers, confirmed by immunobiochemical analysis. Electron-microscopic analysis revealed the presence of CD9- or CD81-incorporated extracellular structures in those bilayers. Finally, microinjection of in vitro-synthesized RNA showed that CD9 reversed a fusion defect in CD81-deficient oocytes in addition to CD9-deficient oocytes, but CD81 failed in both oocytes. These results suggest that both CD9 and CD81 independently work upon sperm-oocyte fusion as extracellular components.
- Subjects :
- QH301-705.5
Science
Lipid bilayer fusion
Membrane fusion
Oocyte activation
Biology
CD9
Oocyte
Bioinformatics
Sperm
General Biochemistry, Genetics and Molecular Biology
Cell biology
Cell membrane
Exosome
medicine.anatomical_structure
CD81
Tetraspanin
embryonic structures
medicine
Extracellular
Biology (General)
General Agricultural and Biological Sciences
Microinjection
Research Article
Subjects
Details
- ISSN :
- 20466390
- Volume :
- 1
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Biology open
- Accession number :
- edsair.doi.dedup.....6168d6ec296665f83c942a7945ea104a