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Molecular tests to assign individuals within the Cacospylla pruni complex
- Source :
- PLoS ONE, PLoS ONE, Public Library of Science, 2013, 8 (8), pp.e72454. ⟨10.1371/journal.pone.0072454⟩, Plos One 8 (8), . (2013), PLoS ONE, Vol 8, Iss 8, p e72454 (2013)
- Publication Year :
- 2013
- Publisher :
- HAL CCSD, 2013.
-
Abstract
- BGPI : équipe 6; International audience; Crop protection requires the accurate identification of disease vectors, a task that can be made difficult when these vectors encompass cryptic species. Here we developed a rapid molecular diagnostic test to identify individuals of Cacopsylla pruni (Scopoli, 1763) (Hemiptera: Psyllidae), the main vector of the European stone fruit yellows phytoplasma. This psyllid encompasses two highly divergent genetic groups that are morphologically similar and that are characterized by genotyping several microsatellite markers, a costly and time-consuming protocol. With the aim of developing species-specific PCR primers, we sequenced the Internal Transcribed Spacer 2 (ITS2) on a collection of C. pruni samples from France and other European countries. ITS2 sequences showed that the two genetic groups represent two highly divergent clades. This enabled us to develop specific primers for the assignment of individuals to either genetic group in a single PCR, based on ITS2 amplicon size. All previously assigned individuals yielded bands of expected sizes, and the PCR proved efficient on a larger sample of 799 individuals. Because none appeared heterozygous at the ITS2 locus (i.e., none produced two bands), we inferred that the genetic groups of C. pruni, whose distribution is partly sympatric, constitute biological species that have not exchanged genes for an extended period of time. Other psyllid species (Cacopsylla, Psylla, Triozidae and Aphalaridae) failed to yield any amplicon. These primers are therefore unlikely to produce false positives and allow rapid assignment of C. pruni individuals to either cryptic species.
- Subjects :
- 0106 biological sciences
Species complex
food.ingredient
Cacopsylla
Molecular Sequence Data
lcsh:Medicine
Biology
european stone fruit yellows
Polymerase Chain Reaction
010603 evolutionary biology
01 natural sciences
Hemiptera
food
Species Specificity
[SDV.SA.STA]Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of agriculture
DNA, Ribosomal Spacer
Animals
cacopsylla pruni
Internal transcribed spacer
lcsh:Science
european stone fruit yellows phytoplasma
Phylogeny
DNA Primers
Genetics
cryptic species
Binding Sites
Vegetal Biology
Multidisciplinary
Base Sequence
Geography
lcsh:R
Amplicon
biology.organism_classification
Amplicon Size
010602 entomology
[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics
Psylla
Genetic Loci
Microsatellite
lcsh:Q
Triozidae
Biologie végétale
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, PLoS ONE, Public Library of Science, 2013, 8 (8), pp.e72454. ⟨10.1371/journal.pone.0072454⟩, Plos One 8 (8), . (2013), PLoS ONE, Vol 8, Iss 8, p e72454 (2013)
- Accession number :
- edsair.doi.dedup.....6158f9c2910b7b66e97582957885cc46