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CD24hiCD27+B cells from patients with allergic asthma have impaired regulatory activity in response to lipopolysaccharide
- Source :
- Clinical and Experimental Allergy, 44(4), 517-528
- Publication Year :
- 2014
- Publisher :
- Wiley, 2014.
-
Abstract
- Background Regulatory B cells have been identified that strongly reduce allergic and auto-immune inflammation in experimental models by producing IL-10. Recently, several human regulatory B-cell subsets with an impaired function in auto-immunity have been described, but there is no information on regulatory B cells in allergic asthma. Objective In this study, the frequency and function of IL-10 producing B-cell subsets in allergic asthma were investigated. Methods Isolated peripheral blood B cells from 13 patients with allergic asthma and matched healthy controls were analyzed for the expression of different regulatory B-cell markers. Next, the B cells were activated by lipopolysaccharide (LPS), CpG or through the B-cell receptor, followed by co-culture with endogenous memory CD4(+) T cells and house dust mite allergen DerP1. Results Lower number of IL-10 producing B cells were found in patients in response to LPS, however, this was not the case when B cells were activated through the B-cell receptor or by CpG. Further dissection showed that only the CD24(hi)CD27(+) B-cell subset was reduced in number and IL-10 production to LPS. In response to DerP1, CD4(+) T cells from patients co-cultured with LPS-primed total B cells produced less IL-10 compared to similar cultures from controls. These results are in line with the finding that sorted CD24(hi)CD27(+) B cells are responsible for the induction of IL-10(+) CD4(+) T cells. Conclusions Taken together, these data indicate that CD24(hi)CD27(+) B cells from allergic asthma patients produce less IL-10 in response to LPS leading to a weaker IL-10 induction in T cells in response to DerP1, which may play a role in allergic asthma.
- Subjects :
- Adult
Lipopolysaccharides
Male
Allergy
Lipopolysaccharide
Regulatory B cells
Immunology
B-Lymphocyte Subsets
Inflammation
Lymphocyte Activation
Immunophenotyping
Young Adult
chemistry.chemical_compound
Risk Factors
T-Lymphocyte Subsets
medicine
Humans
Immunology and Allergy
human
Lymphocyte Count
Receptor
Asthma
CD24
business.industry
CD24 Antigen
Middle Aged
allergy
regulatory B cells
medicine.disease
Interleukin-10
Respiratory Function Tests
Tumor Necrosis Factor Receptor Superfamily, Member 7
Toll-Like Receptor 4
Interleukin 10
Phenotype
chemistry
Case-Control Studies
IL-10
Female
medicine.symptom
business
Subjects
Details
- ISSN :
- 09547894
- Volume :
- 44
- Database :
- OpenAIRE
- Journal :
- Clinical & Experimental Allergy
- Accession number :
- edsair.doi.dedup.....613e2373a677e3c8f93d245cc604c4b0