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Transgenic zebrafish for detecting mutations caused by compounds in aquatic environments
- Source :
- Nature biotechnology. 18(1)
- Publication Year :
- 2000
-
Abstract
- We have established a transgenic zebrafish line carrying a shuttle vector plasmid (pML4) for detecting mutagens in aquatic environments. The plasmid contains the rpsL gene of Escherichia coli as a mutational target gene, and the kanamycin-resistance gene for recovering the plasmid from the chromosomal DNA. To evaluate the system, we treated embryos of the transgenic fish with N-ethyl-N-nitrosourea (ENU), which induces a dose-dependent increase in the mutation frequency of the target gene. The mutation spectrum was consistent with the proposed mechanism of ENU mutagenesis. Similarly, treating the embryos with benzo[a]pyrene or 2-amino-3, 8-dimethylimidazo[4,5- f]quinoxaline, which are found in naturally polluted water, significantly increased the frequency of mutations in the target gene.
- Subjects :
- Male
Transgene
DNA Mutational Analysis
Genetic Vectors
Biomedical Engineering
Gene Dosage
Mutagenesis (molecular biology technique)
Bioengineering
Biology
medicine.disease_cause
Applied Microbiology and Biotechnology
Gene dosage
Animals, Genetically Modified
Plasmid
Shuttle vector
Quinoxalines
medicine
Benzo(a)pyrene
Animals
Transgenes
Mutation frequency
Gene
Zebrafish
Genetics
Mutation
Dose-Response Relationship, Drug
Mutagenicity Tests
Ribosomal Protein S9
Escherichia coli Proteins
Molecular biology
Mutagenesis
Ethylnitrosourea
Molecular Medicine
Female
Water Pollutants, Chemical
Biotechnology
Mutagens
Subjects
Details
- ISSN :
- 10870156
- Volume :
- 18
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Nature biotechnology
- Accession number :
- edsair.doi.dedup.....613e10e13a5f70d64c82cb8b66d8fa4b