Back to Search
Start Over
Occurrence and Characterization ofCronobacterspp. in Powdered Formula from Chinese Retail Markets
- Source :
- Foodborne Pathogens and Disease. 11:307-312
- Publication Year :
- 2014
- Publisher :
- Mary Ann Liebert Inc, 2014.
-
Abstract
- Cronobacter spp. (formerly known as Enterobacter sakazakii) are foodborne pathogens that cause rare but life-threatening diseases in neonates and infants through consumption of contaminated powdered infant formula. This study was conducted to investigate the occurrence of Cronobacter spp. in powdered formula in China and to further characterize Cronobacter isolates. Isolates were identified to the species level based on the fusA gene sequence, and strains of C. sakazakii were further subtyped by applying the polymerase chain reaction (PCR)-based serotyping method. A total of 23 strains of Cronobacter spp. isolated from 530 powdered formula samples were identified using conventional biochemical methods and duplex PCR. Cronobacter spp. were detected in 6.25%, 1.82%, 3.64%, 5.45%, and 2.50% of the general formula, infant formula (age6 months), follow-up formula (6-12 months of age), growing-up formula (1-3 years of age), and children's formula (3-6 years of age), respectively. The individual species were identified as C. sakazakii (22 isolates) and C. malonaticus (1 isolate). Among 22 C. sakazakii isolates, representatives of all but two O-antigen serotypes (serotypes O5 and O6) were recognized.
- Subjects :
- Serotype
China
Molecular Sequence Data
Serogroup
Applied Microbiology and Biotechnology
Microbiology
law.invention
Cronobacter sakazakii
Species level
law
Humans
Food science
Cronobacter
Alleles
Polymerase chain reaction
DNA Primers
Base Sequence
biology
Enterobacteriaceae Infections
Genetic Variation
Infant
Sequence Analysis, DNA
Enterobacter
Peptide Elongation Factor G
biology.organism_classification
Infant Formula
Duplex pcr
Infant formula
Consumer Product Safety
Food Microbiology
Animal Science and Zoology
Gene sequence
Multiplex Polymerase Chain Reaction
Food Science
Subjects
Details
- ISSN :
- 15567125 and 15353141
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Foodborne Pathogens and Disease
- Accession number :
- edsair.doi.dedup.....6110f588ddd0bac27890186915f21d6b