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Intra-spindle Microtubule Assembly Regulates Clustering of Microtubule-Organizing Centers during Early Mouse Development
- Source :
- Cell Reports, Vol 15, Iss 1, Pp 54-60 (2016)
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- SummaryErrors during cell division in oocytes and early embryos are linked to birth defects in mammals. Bipolar spindle assembly in early mouse embryos is unique in that three or more acentriolar microtubule-organizing centers (MTOCs) are initially formed and are then clustered into two spindle poles. Using a knockout mouse and live imaging of spindles in embryos, we demonstrate that MTOC clustering during the blastocyst stage requires augmin, a critical complex for MT-dependent MT nucleation within the spindle. Functional analyses in cultured cells with artificially increased numbers of centrosomes indicate that the lack of intra-spindle MT nucleation, but not loss of augmin per se or overall reduction of spindle MTs, is the cause of clustering failure. These data suggest that onset of mitosis with three or more MTOCs is turned into a typical bipolar division through augmin-dependent intra-spindle MT assembly.
- Subjects :
- 0301 basic medicine
Cell division
Mitosis
Biology
Microtubules
General Biochemistry, Genetics and Molecular Biology
Spindle pole body
MTOC clustering
03 medical and health sciences
Mice
0302 clinical medicine
Microtubule
Live cell imaging
Animals
early mouse development
lcsh:QH301-705.5
Cells, Cultured
Microtubule organizing center
spindle
Cell biology
Spindle apparatus
γ-tubulin complex
030104 developmental biology
Blastocyst
lcsh:Biology (General)
Centrosome
augmin
Microtubule-Associated Proteins
030217 neurology & neurosurgery
Microtubule-Organizing Center
Subjects
Details
- ISSN :
- 22111247
- Volume :
- 15
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Cell Reports
- Accession number :
- edsair.doi.dedup.....6110562d1d10209c4bbe15885b78ec08
- Full Text :
- https://doi.org/10.1016/j.celrep.2016.02.087