Back to Search
Start Over
Molecular characterization of chicken prion proteins by C-terminal-specific monoclonal antibodies
- Source :
- Veterinary immunology and immunopathology. 128(4)
- Publication Year :
- 2008
-
Abstract
- We obtained seven monoclonal antibodies (mAbs) against chicken cellular prion protein (ChPrP C ) by immunizing BALB/c mice with recombinant prion protein (rChPrP). Of the seven mAbs, two mAbs (6 and 26) could recognize rChPrP, but not ChPrP C , in chicken brain lysate via Western blot (WB) analysis. Three C-terminal linear epitopes (AAANQTEVEM, RWWS and SPVPQD) were identified in ChPrP amino acids by pepspot analysis with five mAbs. The mAbs recognizing the C-terminal epitopes in ChPrP C predominantly reacted with the N-terminal truncated ChPrP C in WB analysis, which differed from the reaction with N-terminal proline/glycine-rich repeats recognizing rabbit polyclonal antibody. These mAbs will soon be available as a useful tool to characterize the biology of ChPrP C in birds.
- Subjects :
- medicine.drug_class
Prions
Immunology
Blotting, Western
Enzyme-Linked Immunosorbent Assay
Chick Embryo
Monoclonal antibody
Epitope
law.invention
Prion Diseases
Epitopes
Mice
Western blot
law
medicine
Animals
Poultry Diseases
chemistry.chemical_classification
Mice, Inbred BALB C
General Veterinary
biology
medicine.diagnostic_test
Antibodies, Monoclonal
Molecular biology
Amino acid
chemistry
Polyclonal antibodies
Glycine
biology.protein
Recombinant DNA
Antibody
Chickens
Subjects
Details
- ISSN :
- 01652427
- Volume :
- 128
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Veterinary immunology and immunopathology
- Accession number :
- edsair.doi.dedup.....61104bf56b179b270de0a998f88cac69