Back to Search
Start Over
Region- and sex-specific changes in CART mRNA in rat hypothalamic nuclei induced by forced swim stress
- Source :
- Brain Research. 1479:62-71
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- WOS: 000310414300006<br />PubMed ID: 22960117<br />Cocaine and amphetamine regulated transcript (CART) mRNA and peptides are highly expressed in the paraventricular (PVN), dorsomedial (DMH) and arcuate (ARC) nuclei of the hypothalamus. It has been suggested that these nuclei regulate the hypothalamic-pituitary-adrenal (HPA) axis, autonomic nervous system activity, and feeding behavior. Our previous studies showed that forced swim stress augmented CART peptide expression significantly in whole hypothalamus of male rats. In another study, forced swim stress increased the number of CART-immunoreactive cells in female PVN, whereas no effect was observed in male PVN or in the ARC nucleus of either sex. In the present study, we evaluated the effect of forced swim stress on CART mRNA expression in PVN, DMH and ARC nuclei in both male and female rats. Twelve male (stressed and controls, n=6 each) and 12 female (stressed and controls, n=6 each) Sprague-Dawley rats were used. Control animals were only handled, whereas forced swim stress procedure was applied to the stressed groups. Brains were dissected and brain sections containing PVN, DMH and ARC nuclei were prepared. CART mRNA levels were determined by in situ hybridization. In male rats, forced swim stress upregulated CART mRNA expression in DMH and downregulated it in the ARC. In female rats, forced swim stress increased CART mRNA expression in PVN and DMH, whereas a decrease was observed in the ARC nucleus. Our results show that forced swim stress elicits region- and sex-specific changes in CART mRNA expression in rat hypothalamus that may help in explaining some of the effects of stress. (C) 2012 Elsevier B.V. All rights reserved.<br />NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [3 R01 DA010732-05S1]; National Center for Research ResourcesUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Center for Research Resources (NCRR) [P51RR165]; Office of Research Infrastructure Programs/OD [P51OD11132]<br />This study was supported by NIH Grant no. 3 R01 DA010732-05S1 and by the National Center for Research Resources (P51RR165) and is currently supported by the Office of Research Infrastructure Programs/OD (P51OD11132).
- Subjects :
- Male
Cart
endocrine system
medicine.medical_specialty
Hypothalamus
Dorsomedial Hypothalamic Nucleus
Nerve Tissue Proteins
In situ hybridization
Biology
Stress
Article
Cocaine and amphetamine regulated transcript
Rats, Sprague-Dawley
Internal medicine
medicine
CART
Animals
RNA, Messenger
Molecular Biology
Swimming
Sex Characteristics
Messenger RNA
Arc (protein)
General Neuroscience
Arcuate Nucleus of Hypothalamus
Rats
Autonomic nervous system
Endocrinology
medicine.anatomical_structure
nervous system
Female
Neurology (clinical)
Nucleus
Stress, Psychological
hormones, hormone substitutes, and hormone antagonists
Paraventricular Hypothalamic Nucleus
Developmental Biology
Subjects
Details
- ISSN :
- 00068993
- Volume :
- 1479
- Database :
- OpenAIRE
- Journal :
- Brain Research
- Accession number :
- edsair.doi.dedup.....6109781d55ac398bb8ead230d13a0f17