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Influence of interleukin 1 beta and tumour necrosis factor alpha on the in vitro growth, maturation and mitochondrial distribution of bovine oocytes from small antral follicles
Influence of interleukin 1 beta and tumour necrosis factor alpha on the in vitro growth, maturation and mitochondrial distribution of bovine oocytes from small antral follicles
- Source :
- Zygote (Cambridge, England). 26(5)
- Publication Year :
- 2018
-
Abstract
- SummaryThis study aimed to investigate the effects of IL1β and TNFα on growth and maturation of oocytes from small follicles (1–3 mm) during in vitro culture. To this end, cumulus–oocyte complexes (COCs) with diameters of ~110 µm were cultured in TCM-199 medium alone or supplemented with IL1β (10 ng/ml), TNFα (10 ng/ml) or both for 48 h. The oocytes were measured at the beginning and at the end of the culture period. COCs were cultured for 20 h in pre-maturation medium and then half of the COCs of each group was destined for in vitro maturation and the remaining COCs were used to evaluate meiotic progression, mitochondrial distribution and the expression of mRNAs for GDF-9, c-Mos, Cyclin-B1 and H1foo. The results showed that COCs cultured with TNFα alone or together with IL1β had higher diameters than those cultured in control medium alone or supplemented with IL1β. Control oocytes isolated from large antral follicles (>5 mm) had heterogeneous distribution of mitochondria. Oocytes isolated from small antral follicles, that had been grown in vitro in TCM-199 alone or supplemented with TNFα had similar heterogeneous mitochondrial distribution before in vitro maturation (IVM). After IVM, mitochondria were heterogeneously distribution when cultured in TCM-199. However, when cultured with TNFα and/or IL1β, mitochondria were homogeneously distributed. Presence of TNFα and/or IL1β in TCM-199 culture medium did not influence the expression of mRNAs for GDF-9, c-Mos, Cyclin-B1 and H1foo. In conclusion, TNFα and a mixture of TNFα and IL1β both stimulated the growth of bovine oocytes during their in vitro culture, but do not influence gene expression in grown oocytes.
- Subjects :
- 0301 basic medicine
Interleukin-1beta
Growth Differentiation Factor 9
Mitochondrion
Andrology
03 medical and health sciences
Ovarian Follicle
Gene expression
Distribution (pharmacology)
Animals
Cyclin B1
Cells, Cultured
Chemistry
Tumor Necrosis Factor-alpha
Cell Biology
Antral follicle
In vitro
In vitro maturation
H1FOO
In Vitro Oocyte Maturation Techniques
Mitochondria
030104 developmental biology
Gene Expression Regulation
Proto-Oncogene Proteins c-mos
Oocytes
Tumor necrosis factor alpha
Cattle
Female
Developmental Biology
Subjects
Details
- ISSN :
- 14698730
- Volume :
- 26
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Zygote (Cambridge, England)
- Accession number :
- edsair.doi.dedup.....60e6bc75d799f27df08cfc645491c0df