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Deciphering Posttranslational Processing Events in the Pituitary of a Neopterygian Fish: Cloning of a Gar Proopiomelanocortin cDNA
- Source :
- General and Comparative Endocrinology. 107:401-413
- Publication Year :
- 1997
- Publisher :
- Elsevier BV, 1997.
-
Abstract
- A cDNA that codes for the polypeptide hormone precursor proopiomelanocortin (POMC) was cloned and sequenced from a gar (Lepisosteus osseus) pituitary cDNA library. The gar POMC cDNA is 1237 bp and contains a 780-bp open reading frame. The deduced amino acid sequence for gar POMC is 259 amino acids in length. The general organization of gar POMC is very similar to that of other gnathostome POMC sequences. The beta-endorphin sequence had 91% sequence identity with sockeye A beta-endorphin and 71% sequence identity with Xenopus laevis beta-endorphin. Three melanocyte-stimulating hormone (MSH) core sequences [HFR(W)] were detected. The gar alpha-MSH sequence was identical to the alpha-MSH sequence in rat POMC. The gar beta-MSH sequence had 77% sequence identity with salmonid forms of beta-MSH and 53% sequence identity with tetrapod forms of beta-MSH. The gamma-MSH region of gar POMC only had 26% primary sequence identity with tetrapod gamma-MSH sequences. Gar gamma-MSH had an incomplete MSH core sequence (HRF), an apparent internal deletion of five amino acids, and lacked flanking paired basic amino acids essential for proteolytic cleavage. The apparent degenerate nature of gar gamma-MSH is discussed in light of the absence of this sequence in salmonid fish.
- Subjects :
- Male
endocrine system
Pro-Opiomelanocortin
animal structures
Molecular Sequence Data
Peptide hormone
Biology
Polymerase Chain Reaction
Endocrinology
Species Specificity
Proopiomelanocortin
Complementary DNA
Animals
Amino Acid Sequence
Melanocyte-Stimulating Hormones
Cloning, Molecular
Peptide sequence
Cloning
chemistry.chemical_classification
Genetics
Base Sequence
integumentary system
cDNA library
beta-Endorphin
digestive, oral, and skin physiology
Fishes
DNA
Amino acid
Open reading frame
chemistry
Pituitary Gland
biology.protein
RNA
Female
Animal Science and Zoology
Protein Processing, Post-Translational
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- ISSN :
- 00166480
- Volume :
- 107
- Database :
- OpenAIRE
- Journal :
- General and Comparative Endocrinology
- Accession number :
- edsair.doi.dedup.....60e3a16f431ba8feb622fa5185e1a9da