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Effects of reactive oxygen species generation induced by Wonju City particulate matter on mitochondrial dysfunction in human middle ear cell
- Source :
- Environmental Science and Pollution Research. 28:49244-49257
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Atmospheric particulate matter (PM) contains different components that can elicit varying adverse health effects in humans and animals. Studies on PM toxicity and its underlying mechanisms in the middle ear are limited, and they generally use a PM standard. However, as PM composition varies temporally and geographically, it is crucial to identify the toxic PM constituents according to season and region and investigate their associated health effects. Thus, we sought to determine whether PM induces cytotoxicity and inflammatory factor and reactive oxygen species (ROS) generation in human middle ear epithelial cells obtained from patients with otitis media. The cells were treated with both standard urban PM and PM directly captured from the atmosphere in Wonju City. The association between mitochondrial dysfunction and PM was investigated. PM exposure significantly increased COX-2 and TNF-α mRNA expression, increased ROS generation, induced inflammatory responses, and caused abnormalities in mitochondrial motility and function. Furthermore, PM induced cell apoptosis, which consequently reduced cell survival, particularly at the concentration of 100 μg/mL. Overall, our study provides new insights into the toxic effects of standard and atmospheric PM on middle ear cell line.
- Subjects :
- medicine.medical_specialty
Health, Toxicology and Mutagenesis
Cell
Ear, Middle
Motility
Inflammation
010501 environmental sciences
medicine.disease_cause
01 natural sciences
Internal medicine
medicine
Animals
Humans
Environmental Chemistry
Cities
Cytotoxicity
0105 earth and related environmental sciences
chemistry.chemical_classification
Air Pollutants
Reactive oxygen species
General Medicine
Pollution
Mitochondria
Oxidative Stress
Endocrinology
medicine.anatomical_structure
chemistry
Apoptosis
Toxicity
Particulate Matter
medicine.symptom
Reactive Oxygen Species
Oxidative stress
Subjects
Details
- ISSN :
- 16147499 and 09441344
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Environmental Science and Pollution Research
- Accession number :
- edsair.doi.dedup.....60e04cb2d647db3ea8457c83d2c75f1c