Back to Search
Start Over
Immune responses in multiple hosts to Nucleocapsid Protein (NP) of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)
- Source :
- PLoS Neglected Tropical Diseases, PLoS Neglected Tropical Diseases, Vol 15, Iss 12, p e0009973 (2021)
- Publication Year :
- 2021
- Publisher :
- Public Library of Science, 2021.
-
Abstract
- In 2019, the World Health Organization declared 3 billion to be at risk of developing Crimean Congo Hemorrhagic Fever (CCHF). The causative agent of this deadly infection is CCHFV. The data related to the biology and immunology of CCHFV are rather scarce. Due to its indispensable roles in the viral life cycle, NP becomes a logical target for detailed viral immunology studies. In this study, humoral immunity to NP was investigated in CCHF survivors, as well as in immunized mice and rabbits. Abundant antibody response against NP was demonstrated both during natural infection in humans and following experimental immunizations in mice and rabbits. Also, cellular immune responses to recombinant NP (rNP) was detected in multispecies. This study represents the most comprehensive investigation on NP as an inducer of both humoral and cellular immunity in multiple hosts and proves that rNP is an excellent candidate warranting further immunological studies specifically on vaccine investigations.<br />Author summary Crimean Congo Hemorrhagic Fever Virus (CCHFV) is the most lethal human pathogen of medical importance after the dengue virus among arboviruses. The increasing geographic spread of Hyalomma ticks, which are responsible for viral transmission widespread, threatens billions of people. WHO currently declares the field of research on CCHFV as the second most urgently needed areas of investigations on emerging pathogens. About 10 to 40% of those infected with the virus lose their life due to the rapidly developing severe clinical manifestations. Pandemic potential and the lack of any approved treatment or vaccine make raise the studies on CCHFV as critical. The studies on CCHFV are challenging due to the necessities of BSL-4 facilities and the immunological characterization of individual structural proteins will lay the groundwork for the steps to be taken to treat and prevent this emerging disease. As is known from other RNA viruses, nucleoprotein (NP) has crucial roles in the viral life cycle, both in viral replication and transcription and in the formation of the virion structure. So far, detailed and comprehensive immunological characterizations on NP in multiple are not undertaken. Our study was set out to embark such detailed investigation. The strong humoral and cellular immune response to NP demonstrated by this study indicates that NP might be an excellent candidate for future scrutinies on vaccines and diagnostic reagents.
- Subjects :
- Crimean–Congo hemorrhagic fever
Male
Cellular immunity
Physiology
RC955-962
Antibodies, Viral
Biochemistry
law.invention
Cell-Mediated Immunity
White Blood Cells
Mice
law
Animal Cells
Arctic medicine. Tropical medicine
Immune Physiology
Medicine and Health Sciences
Lymphocytes
Enzyme-Linked Immunoassays
Immune Response
Mammals
Innate Immune System
Mice, Inbred BALB C
Immune System Proteins
Eukaryota
Animal Models
Nucleocapsid Proteins
Infectious Diseases
Ribonucleoproteins
Experimental Organism Systems
Vertebrates
Hemorrhagic Fever Virus, Crimean-Congo
Recombinant DNA
Leporids
Cytokines
Rabbits
Public aspects of medicine
RA1-1270
Cellular Types
Crimean Congo hemorrhagic fever virus
Research Article
Crimean-Congo Hemorrhagic Fever Virus (CCHFV)
Nucleocapsid Protein (NP)
Immune Cells
Immunology
Biology
Research and Analysis Methods
World health
Antibodies
Immune system
Viral life cycle
medicine
Animals
Humans
Immunoassays
Blood Cells
Public Health, Environmental and Occupational Health
Immunity
Organisms
Biology and Life Sciences
Proteins
Cell Biology
Molecular Development
medicine.disease
Virology
Immunity, Humoral
Immune System
Humoral immunity
Amniotes
Animal Studies
Immunologic Techniques
Hemorrhagic Fever, Crimean
Immunization
Zoology
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19352735 and 19352727
- Volume :
- 15
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- PLoS Neglected Tropical Diseases
- Accession number :
- edsair.doi.dedup.....60d6d70a6119bf4df0e9dd198a7215da