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Erythropoietin Markedly Attenuates Brain Infarct Size and Improves Neurological Function in the Rat
- Source :
- Journal of Investigative Medicine. 58:893-904
- Publication Year :
- 2010
- Publisher :
- SAGE Publications, 2010.
-
Abstract
- Background The impact of epoetin beta (recombinant human erythropoietin) on brain infarction area (BIA) and neurological status in a rat model of acute ischemic stroke (IS) induced by distal left internal carotid artery occlusion was investigated. Methods Adult male Sprague-Dawley rats (n = 30) were categorized into group 2 (IS only) and group 3 (IS plus intraperitoneal erythropoietin 5000 IU/kg at 0, 12, and 24 hours after IS). Healthy Sprague-Dawley rats (n = 10) served as group 1. Results Analysis of brain tissues showed larger BIA in group 2 than in group 3 ( P < 0.001). Corner test identified highest frequency of left turn in group 2 ( P < 0.05). The mRNA expressions of Bax, caspase 3, interleukin 18, toll-like receptor 4, and plasminogen activator inhibitor 1 were highest, whereas Bcl-2 was lowest in group 2 ( P < 0.05). Lower CXCR4 and stromal cell-derived factor 1 expressions were noted in group 2 than in group 3 ( P < 0.01). Immunohistofluorescence staining showed lower expressions of CXCR4, stromal cell-derived factor 1, von Willebrand factor, and doublecortin with higher number of apoptotic nuclei in group 2 than in group 3 ( P < 0.001). Immunohistochemical staining demonstrated lower cellular proliferation and number of small vessels with higher glial fibrillary acid protein expression in group 2 than in group 3 ( P < 0.01). Conclusions Erythropoietin significantly limited BIA and improved sensorimotor dysfunction after acute IS.
- Subjects :
- Brain Infarction
Male
medicine.medical_specialty
Doublecortin Protein
Neurogenesis
Neurological function
Urology
Neovascularization, Physiologic
Apoptosis
General Biochemistry, Genetics and Molecular Biology
Brain Ischemia
Rats, Sprague-Dawley
Neovascularization
Occlusion
medicine
Animals
Humans
Erythropoietin
Acute ischemic stroke
Epoetin beta
business.industry
Neurological status
Recovery of Function
General Medicine
Recombinant Proteins
Rats
Disease Models, Animal
Oxidative Stress
Brain infarction
Anesthesia
Acute Disease
Hematinics
Encephalitis
medicine.symptom
business
medicine.drug
Subjects
Details
- ISSN :
- 17088267 and 10815589
- Volume :
- 58
- Database :
- OpenAIRE
- Journal :
- Journal of Investigative Medicine
- Accession number :
- edsair.doi.dedup.....60d68e26422dcb9f57c1ecd1a81188f2