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Nanostructured polymeric coatings based on chitosan and dopamine-modified hyaluronic acid for biomedical applications
- Source :
- RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia, instname, Small, Small, Wiley-VCH Verlag, 2014, 10 (12), pp.2459-2469. ⟨10.1002/smll.201303568⟩, Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Publication Year :
- 2014
- Publisher :
- John Wiley & Sons, 2014.
-
Abstract
- In a marine environment, specific proteins are secreted by mussels and used as a bioglue to stick to a surface. These mussel proteins present an unusual amino acid 3,4-dihydroxyphenylalanine (known as DOPA). The outstanding adhesive properties of these materials in the sea harsh conditions have been attributed to the presence of the catechol groups present in DOPA. Inspired by the structure and composition of these adhesive proteins, we used dopamine-modified hyaluronic acid (HA-DN) prepared by carbodiimide chemistry to form thin and surface-adherent dopamine films. This conjugate was characterized by distinct techniques, such as nuclear magnetic resonance and ultraviolet spectrophotometry. Multilayer films were developed based on chitosan and HA-DN to form polymeric coatings using the layer-by-layer methodology. The nanostructured films formation was monitored by quartz crystal microbalance. The film surface was characterized by atomic force microscopy and scanning electron microscopy. Water contact angle measurements were also conducted. The adhesion properties were analyzed showing that the nanostructured films with dopamine promote an improved adhesion. In vitro tests showed an enhanced cell adhesion, proliferation and viability for the biomimetic films with catechol groups, demonstrating their potential to be used in distinct biomedical applications.<br />The authors want to acknowledge the COST Action TD0906 - Biological adhesives: from biology to biomimetics. The authors also acknowledge the financial support from the Fundacao para a Ciencia e para a Tecnologia through the Ph.D. grants with the references SFRH/BD/73119/2010 and SFRH/BD/69529/2010. G. G. Ferrer acknowledges the support of the Spanish Ministry of Science and Innovation for the mobility grant JC2008-00135. G. Botelho acknowledges the NMR portuguese network (PTNMR, Bruker Avance III 400-Univ. Minho).
- Subjects :
- [SDV.BIO]Life Sciences [q-bio]/Biotechnology
Polymers
Dopamine
Multilayer films
Microscopy, Atomic Force
Chitosan
Contact angle
chemistry.chemical_compound
Mice
Surface modification
Coated Materials, Biocompatible
Biomimetics
Materials Testing
General Materials Science
[INFO.INFO-BT]Computer Science [cs]/Biotechnology
Hyaluronic Acid
ComputingMilieux_MISCELLANEOUS
Cells, Cultured
chemistry.chemical_classification
Tissue Scaffolds
[CHIM.MATE]Chemical Sciences/Material chemistry
Adhesion
Polymer
Quartz Crystal Microbalance Techniques
Marine mussels
MAQUINAS Y MOTORES TERMICOS
Biotechnology
Materials science
Surface Properties
Biomaterials
Polymer chemistry
Cell Adhesion
Animals
Humans
14. Life underwater
Science & Technology
Membranes, Artificial
General Chemistry
Quartz crystal microbalance
Nanostructures
chemistry
Chemical engineering
13. Climate action
Microscopy, Electron, Scanning
Adhesive
Subjects
Details
- Language :
- English
- ISSN :
- 16136810 and 16136829
- Database :
- OpenAIRE
- Journal :
- RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia, instname, Small, Small, Wiley-VCH Verlag, 2014, 10 (12), pp.2459-2469. ⟨10.1002/smll.201303568⟩, Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Accession number :
- edsair.doi.dedup.....60c72066aacdd28866db83d3849f72cf